We narrowed to 11,585 results for: Vars;

1. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. medSIIN

   Plasmid

   #185664

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   

   Use

   Lentiviral

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-Bnip3FL

   Plasmid

   #100796

   Purpose

   Mammalian expression of Bnip3 full length splice variant

   Depositor

   Joseph Gordon

   Article

   Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

   Insert

   mus Bnip3 (Bnip3 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV gfaABC1D NES-jRCaMP1a

   Plasmid

   #171120

   Purpose

   Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

   Depositor

   Thomas Oertner

   Article

   Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

   Insert

   jRCaMP1a
   

   Use

   AAV

   Tags

   His Tag, T7 Tag, XpressTag

   Expression

   Mammalian

   Promoter

   gfaABC1D

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGG043

   Plasmid

   #133123

   Purpose

   Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   PsNifH Synthetic consensus nifH promoter
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-haA3A-CBE-G-NG

   Plasmid

   #220897

   Purpose

   Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-G-NG
   

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-HA-mRFP-pcDNA3.1-

   Plasmid

   #52510

   Purpose

   expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET51b-His-TEV-CLIP-tag_fast

   Plasmid

   #167275

   Purpose

   Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIPf-tag
   

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. hiSIIN

   Plasmid

   #185662

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   

   Use

   Lentiviral

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. midSIIN

    Plasmid

    #185665

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. dimSIIN

    Plasmid

    #185661

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. loSIIN

    Plasmid

    #185663

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE32-pr-mEosFP (mEosFP-V69T)

    Plasmid

    #99213

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEosFP
    

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T for primed c…

    Promoter

    T5

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TMEM16A-I546K-peGFP-N1

    Plasmid

    #228582

    Purpose

    Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
    

    Expression

    Mammalian

    Mutation

    I546K

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-Pax6

    Plasmid

    #32932

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Pax6 (isoform 2, transcript variant 5) (Pax6 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. bdSUMO-E5TAT-GFP-HiBit

    Plasmid

    #170993

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-E5TAT-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP2292

    Plasmid

    #70707

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
    

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Available Since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPICZ-pre-pro-alphaf-Btl2

    Plasmid

    #117664

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rescue Septin 11 mRNA

    Plasmid

    #122022

    Purpose

    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Expression

    Mammalian

    Mutation

    Silent mutations are in 5 consecutive codons (AUU…

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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