335,473 results

1. pcDNA 3.2/V5-DEST RBM14-V5

   Plasmid

   #69823

   Purpose

   Expresses RBM14-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   RNA-binding protein 14 (RBM14 Human)
   

   Use

   Retroviral

   Tags

   v5

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV SYN flex PSAM4 GlyR IRES EGFP (AAV9)

   Viral Prep

   #119741-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

3. AAV SYN flex PSAM4 GlyR IRES EGFP (AAV9)

   Viral Prep

   #119741-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pYLL201

   Plasmid

   #53454

   Purpose

   Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

   Depositor

   Lisa Lyu, Leroy Liu

   Article

   Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

   Insert

   HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV8R-12 Capsid

   Plasmid

   #213968

   Purpose

   AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas13d-dsRBD

   Plasmid

   #154938

   Purpose

   BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-TRE3G-PYL1-sfGFP-PML

   Plasmid

   #167011

   Purpose

   expression of PYL1-SfGFP-PML, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   PML (PML Human)
   

   Use

   Lentiviral

   Tags

   GFP-PML

   Expression

   Mammalian

   Available Since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Plasmid

    #179115

    Purpose

    Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-A11(2-88)-Strep

    Plasmid

    #196214

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHelper_TS_V2_ampR

    Plasmid

    #214467

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Promoter

    XylS / AraC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1_V5_TurboID_FLAG_Nt

    Plasmid

    #194072

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-TurboID-FLAG

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pLenti CMV GFP Neo (657-2)

    Plasmid

    #17447

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hSTING (V155M)

    Plasmid

    #214161

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V155M) (STING1 Human)
    

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR v2 mBAX

    Plasmid

    #129577

    Purpose

    CRISPR deletion of murine BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAX (Bax Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-UCHL3

    Plasmid

    #22564

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    UCHL3 (UCHL3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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