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pKLV-EF1a-BFP-W

Plasmid

#208757

Purpose

Lentiviral vector expressing BFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCPP5371

Plasmid

#128732

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSC142

Plasmid

#122835

Purpose

SUC2 promoter with GreenGate A-B flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SUC2 promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-U6a

Plasmid

#107593

Purpose

An entry vector with U6a promoter driving guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

U6a promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSBDEST.H

Plasmid

#79464

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO CFP

Plasmid

#68489

Purpose

TOPO construct expressing CFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cyan Fluorescent Protein

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-PTet-YFP

Plasmid

#62245

Purpose

used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

YFP

Expression

Bacterial

Promoter

Ptet

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CK(1.3)GW

Plasmid

#27227

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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TLD_010

Plasmid

#19696

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk

Plasmid

#18089

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad Aquifex aeolicus)

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3_pLyn_EGFP

Plasmid

#214605

Purpose

Expresses fluorescent protein EGFP targeted to plasma membrane

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

lyn-EGFP

Tags

lyn targetting

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Gateway Cloning

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B3/4

Plasmid

#175785

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B3 and pAd5-B4.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 10862-18018

Use

Adenoviral and Synthetic Biology

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_4

Plasmid

#130514

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)

Plasmid

#70742

Purpose

1 color weak reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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