We narrowed to 11,822 results for: nts

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pUC57-fsr1

Plasmid

#179866

Purpose

Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

Fusarubin polyketide synthase

Use

Synthetic Biology

Mutation

Codon optimized for expression in Saccharomyces c…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-BVRA

Plasmid

#203480

Purpose

For Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Rattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

Plasmid

#197960

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis) K122I

Plasmid

#195614

Purpose

Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

C2458G, A2826T, A2827T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9994-HypaR661A-ABE

Plasmid

#163002

Purpose

plasmid expressing HypaR661A-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

R661A

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-pleCopyCatcher

Plasmid

#174061

Purpose

CopyCatcher insertion donor for the pale locus

Depositor

Ethan Bier

Article

Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

Insert

Pale (Pale Fly, Synthetic)

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-NbBCP1bPro

Plasmid

#159417

Purpose

Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

NbBCP1b Pro

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE672

Plasmid

#153238

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-TCAP-mKate2

Plasmid

#109496

Purpose

Muscle specific zebrafish expression of mKate2 fused to telethonin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

TCAP (tcap Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p21-P369S-MEFV

Plasmid

#134712

Purpose

Lentiviral expression of human MEFV P369S after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.P369S

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M680I-MEFV

Plasmid

#134711

Purpose

Lentiviral expression of human MEFV M680I after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.M680I

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M694I-MEFV

Plasmid

#134710

Purpose

Lentiviral expression of human MEFV M694I after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.M694I

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M694V-MEFV

Plasmid

#134703

Purpose

Lentiviral expression of human MEFV M694V after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.M694V

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-mTRAF3ip3∆TM-CFP

Plasmid

#125182

Purpose

Expresses mouse TRAF3IP3 ∆TM in mammalian cells

Depositor

Shao-Cong Sun

Insert

TRAF3ip3 (Traf3ip3 Mouse)

Tags

CFP

Expression

Mammalian

Mutation

K87R and S359P- See depositor comments below

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0014

Plasmid

#117550

Purpose

Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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