We narrowed to 92,789 results for: Mal

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

Plasmid

#108735

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA-mCherry

Plasmid

#99878

Purpose

Expression of GFP-AURKA-mCherry in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Mammalian

Promoter

AURKA minimal

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3A

Plasmid

#137756

Purpose

Expression of LC3A

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3A (MAP1LC3A Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC74

Plasmid

#62342

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-E-cadherin promoter

Plasmid

#61798

Purpose

Luciferase reporter containing the mouse E-cadherin promoter

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108770

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

Plasmid

#108737

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8s

Plasmid

#172720

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2*

Plasmid

#169850

Purpose

Mammalian Expression, SpyCas9 prime editor, optimized NLS

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

spCas9 PE*

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA

Plasmid

#108738

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-SunTag-RPS7-PP7-WPRE

Plasmid

#198338

Purpose

PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

GCN4x24-RPS7-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops and SunTag

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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MSP1101

Plasmid

#65773

Purpose

Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, and T1337R mutations in C…

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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