167,948 results

1. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-PDGFRb-IRES2-EGFP

   Plasmid

   #204354

   Purpose

   Expresses wild-type PDGFRb gene.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-miniGFP1-P2A-FusionRed

   Plasmid

   #188732

   Purpose

   Express miniGFP1 and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP1
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. phOct4-EGFP

   Plasmid

   #38776

   Depositor

   Wei Cui

   Article

   Gerrard et al Stem Cells. 2005;23(1):124-33.

   Insert

   Oct-4 promoter (POU5F1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   human OCT4

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

   Plasmid

   #155305

   Purpose

   Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
   

   Use

   Lentiviral

   Tags

   2xNLS-3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TOgw-C-mCherry

   Plasmid

   #223952

   Purpose

   Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEGFP-PLIN3

   Plasmid

   #222426

   Purpose

   To express PLIN3 with mEGFP tag in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   PLIN3 (PLIN3 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-U6+27-Tornado-Pepper-RhoBAST

    Plasmid

    #196343

    Purpose

    Overexpression of circular Pepper-RhoBAST

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    Tornado-Pepper-RhoBAST
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PPRE-H2B-eGFP

    Plasmid

    #84393

    Purpose

    Fluorescent reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid

    #65727

    Purpose

    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Inserts

    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV and UBC

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-TRE-C-prM-E-NS1

    Plasmid

    #175277

    Purpose

    AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Promoter

    tight TRE

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205317

    Purpose

    All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISYmu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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