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pET SUMO-syt7 (136-403) L361C

Plasmid

#213622

Purpose

Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 (136-403) L361C (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

C260A C275A L361C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-Doc2β C2AB (G361C)

Plasmid

#213631

Purpose

Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

Doc2β C2AB (G361C) (Doc2b Rat)

Tags

SUMO

Expression

Bacterial

Mutation

G361C, all original cysteines mutated to alanines

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-HA AXIN1 delta PHGDH (delta 321-340)

Plasmid

#213005

Purpose

expresses mouse AXIN1

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

AXIN1 (Axin1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pS238D1::sptse5-CT_phoA-lacZalpha

Plasmid

#192961

Purpose

Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT_phoA-lacZ_

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317) fused to phoA-lacZal…

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

Plasmid

#162950

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag

Use

Synthetic Biology

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-Myr-TYK2

Plasmid

#139355

Purpose

Expression of membrane-anchored human TYK2 protein fused to GFP

Depositor

Sandra Pellegrini

Article

Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

Insert

TYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus (TYK2 Human)

Tags

aa 1-16 of human Lck and GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Cdk6-T2A-td-sfGFP-FNF-TK

Plasmid

#134321

Purpose

B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Cdk6 (Cdk6 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tandem dimer-superfolder…

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (F317D)

Plasmid

#121406

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (I325D)

Plasmid

#121407

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSN21

Plasmid

#100106

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FN(9-10, RGE)-I27-CC

Plasmid

#85396

Purpose

MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

Depositor

Khalid Salaita

Article

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon

Tags

6x His

Expression

Bacterial

Mutation

GRGDS motif mutated to GRGES

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Btk(PH)Akt1(KD)

Plasmid

#86591

Purpose

Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)

Tags

10xHis

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pC3R112C/T180C

Plasmid

#85088

Purpose

Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The R112C/T180C variant of PCNA3 fused to P450cam

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C in PCNA3

Promoter

T7 promoter

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG250

Plasmid

#80582

Purpose

recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 Erg (317-486)

Plasmid

#66992

Purpose

Bacterial expression plasmid for mouse Erg (317-486) with GST

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (317-486) (Erg Mouse)

Tags

GST fusion

Expression

Bacterial

Mutation

Amino acids from 317-486

Promoter

TAC

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 Erg (209-316)

Plasmid

#66991

Purpose

Bacterial expression plasmid for mouse Erg (209-316) with GST

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (209-316) (Erg Mouse)

Tags

GST fusion

Expression

Bacterial

Mutation

Amino acids from 209-316

Promoter

TAC

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pNB786

Plasmid

#60151

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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tout velou (ttv)

Plasmid

#37401

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

tout-velu (ttv Fly)

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFSW Syt1-C2B V304A,I367A IRES GFP

Plasmid

#133821

Purpose

Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

V304A, I367A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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