We narrowed to 21,576 results for: Cre
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Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET01-TAF5-ex8-Minigene-SRSF1 motif WT
Plasmid#218972PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)DepositorInsertTAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
ExpressionMammalianPromoterRSV LTRAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRM1-DuET
Plasmid#213300PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM1 (GRM1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
eDrEGFR
Plasmid#184000PurposeOptically controlled eDrEGFR expression in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteDrEGFR (EGFR Human)
TagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR
Plasmid#181972PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-D614G
Plasmid#166850PurposeFor expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Spike (S SARS-CoV-2 virus)
TagsC9ExpressionMammalianPromoterCMVAvailable sinceJune 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTet-O-NEUROG3-T2A-PuroR
Plasmid#162341PurposeLentiviral expression of NEUROG3 under the control of the TetON promoterDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-NG-nCas9
Plasmid#163527PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAnc689-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
ExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1
Plasmid#121824PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSa-dCas9/LSD1 (open)
UseGateway Cloning and Synthetic BiologyTagsNLSAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema4c-Fc-His
Plasmid#72156PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-tagBFP
Plasmid#65257Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIi-Streptavidin and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cenp-B INCENP mCherry
Plasmid#45235PurposeTarget INCENP to centromeres via full length CENP-BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCenp-B, INCENP-Δcen, mCherry
Tagsfull length CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt8A-V5
Plasmid#35935DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWnt8A (Wnt8a Human)
TagsV5ExpressionMammalianMutationSilent Mutation A (600) --> GPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)
Plasmid#114087PurposeU6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertentry vector for FnCas12a crRNAs
ExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMH-SFB-DYRK1B
Plasmid#101771PurposeMammalian expression of DYRK1B fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDYRK1B / MIRK (DYRK1B Human)
TagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant alleleAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-miR-216a
Plasmid#65054PurposeExpression of miR216a in mammalian cellsDepositorAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SERA9-bio-His
Plasmid#50820PurposeExpresses enzymatically monobiotinylated full length SERA9DepositorInsertSERA9
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by