We narrowed to 11,662 results for: kars;

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

Plasmid

#20942

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

EF1alpha

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIP-cathepsinD-YMH

Plasmid

#182644

Purpose

Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

cathepsinD

Use

Retroviral

Tags

His6-tag, TS site from proCCK, and myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-hNHERF2

Plasmid

#47801

Purpose

Expresses human NHERF2 in mammalian cells

Depositor

Randy Hall

Article

Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

Insert

NHERF2 (NHERF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pROF021

Plasmid

#155326

Purpose

Expression of PULSE-controlled FLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-FLuc-TSV40

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EGFP-IRES(Neo)R

Plasmid

#158068

Purpose

eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

eGFP

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-4mtGCaMP6f-DIO

Plasmid

#179529

Purpose

Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

4mtGCaMP6f

Use

AAV

Tags

4x Cox8 targeting sequence

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-α-chain

Plasmid

#133730

Purpose

Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

a-Chain_gePSI
ZsGreen1

Expression

Mammalian

Mutation

Only one part of the gePSI-system (aa1-141 + stop…

Promoter

pTRE3G-Bi

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-LAP3

Plasmid

#217660

Purpose

For gateway cloning of LAP3

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

Plasmid

#68280

Purpose

SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Mkk2DS NLS mCherry (MKK2 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

Plasmid

#20943

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1A eIF4A3

Plasmid

#83035

Purpose

mammalian expression of eIF4A3

Depositor

Gideon Dreyfuss

Article

Chan et al RNA. 2004 Feb;10(2):200-9.

Insert

eIF4A3 (EIF4A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only
pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hPLEKHM1

Plasmid

#64146

Purpose

For protein expression in E.coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Promoter

cspA

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only