We narrowed to 30,718 results for: REP

1. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

   Plasmid

   #172647

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172626

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT (PGK) 5xGRE-NanoLuc

   Plasmid

   #207170

   Purpose

   GAl4 response element driven NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

   Inserts

   NanoLuciferase
   d2eGFP
   5x GRE
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Expression

   Mutation

   Promoter

   Gal4 response element minP and PGK

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 GIL-11 myc TEV TwinStrep

   Plasmid

   #199627

   Purpose

   Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

   Depositor

   Jurgen Scheller

   Article

   Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

   Insert

   GIL-11
   

   Use

   Tags

   TwinStrep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Use

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETB-S28 histone 3 phosphorylation reporter

   Plasmid

   #32788

   Depositor

   Alice Ting

   Article

   Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

   Insert

   CFP-14-3-3tau-H3 peptide-YFP
   

   Use

   Tags

   CFP and YFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. Pvalb-2A-Dre Flp-in replacement vector

   Plasmid

   #61571

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-2xFLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172622

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Human, Synthetic)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-PRD(800-868)-strep

    Plasmid

    #180023

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid

    #161587

    Purpose

    expression of Myc-tagged mouse Lrrk2 without GTPase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1023-1879

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid

    #161586

    Purpose

    expression of Myc-tagged mouse Lrrk2 without kinase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1871-2540

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-REPORT(EF1a no GFP) mnuctdTomato

    Plasmid

    #172342

    Purpose

    mnuctdTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Use

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FL MALT1 Catalytic Mutant in pET29b-Strep

    Plasmid

    #48969

    Purpose

    Expresses Full length catalytic mutant MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Catalytic Mutant C464A

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

    Plasmid

    #46670

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-let-7a-1 (MIRLET7A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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