We narrowed to 11,008 results for: T7

Showing: 10481 - 10500 of 11008 results

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pX551-miniCMV-SpCas9D10A nickase

Plasmid

#216735

Purpose

Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

T7 and mini-CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomajRGECO1a

Plasmid

#232842

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted jRGECO1a

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomajRGECO1a

Use

AAV

Tags

nuclear export sequence, 6xHis, T7, Xpress tag

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAC-VIOL

Plasmid

#53087

Purpose

Produces the carotenoid violaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

Insert

zep (ABA1 Mustard Weed)

Use

Low copy number bacterial cloning vector

Mutation

Lacks codons for the first 60 N-terminal amino ac…

Promoter

T7

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0709:Dendra

Plasmid

#159077

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0709

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0919:Dendra

Plasmid

#174936

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0919

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5482:Dendra

Plasmid

#167437

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5482

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Tags

His-tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pT7NT*-Bri2 113-231 R221E

Plasmid

#138134

Purpose

Expresseds human Bri2 BRICHOS R221E mutant in E. coli

Depositor

Jan Johansson

Article

Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

Insert

human Bri2 BRICHOS R221E (ITM2B Human)

Tags

NT* tag derived from spider silk proteins

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Article

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al Commun Biol. 2023 May 15;6(1):526. doi:

Insert

alpha synuclein (SNCA Human)

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCB268

Plasmid

#53363

Purpose

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

Depositor

Alexander Varshavsky

Article

Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

Inserts

DHFR (Dhfr Mouse)
Ub
Aβ42 (APP Human)

Tags

13xMyc, Flag, and HA

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET32bplus_trxAChEwt

Plasmid

#83916

Purpose

Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

Depositor

Sarel Fleishman

Article

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

codon optimised for E.coli expression

Promoter

T7 promoter

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_1353:Dendra

Plasmid

#175002

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_1353

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0250:Dendra

Plasmid

#159047

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0250

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MEK1C222

Plasmid

#164638

Purpose

Bacterial expression plasmid for His6-MEK1C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S222C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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