We narrowed to 3,437 results for: multiplex

Showing: 1041 - 1060 of 3437 results

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pcDNA3-Lyn-sapphireAKAR

Plasmid

#118466

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-sapphireAKAR

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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apt-CBE-3'end

Plasmid

#219939

Purpose

Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_32-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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nCas9-NG_P2A-mCherry

Plasmid

#219934

Purpose

Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry(Y70A)

Plasmid

#209703

Purpose

Mammalian expression of mCherry with Y70A mutation

Depositor

Stewart Loh

Publication

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

mCherry(Y70A)

Expression

Mammalian

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Plasmid

#192124

Purpose

pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Depositor

Wilson Wong

Publication

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCherry-NLS

Plasmid

#186346

Purpose

Nucleus-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-NES

Plasmid

#184453

Purpose

Cytosolic mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-iRFP702-NES

Plasmid

#184457

Purpose

Cytosolic iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLucia

Plasmid

#135287

Purpose

GBPart - Lucia Luciferase CDS

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Lucia Luciferase

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCGB

Plasmid

#135283

Purpose

GBPart - CGB Luciferase CDS

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Click Beetle Green Luciferase

Use

Luciferase and Synthetic Biology

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway Cloning

Tags

tdTomato

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-pac

Plasmid

#165877

Purpose

Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163068

Purpose

Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163071

Purpose

Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaB

Plasmid

#124524

Purpose

pColE1_AlphaB

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mTFP1

Plasmid

#118858

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Publication

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1

Tags

His-tag

Expression

Bacterial

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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