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pEB2-mRFP1

Plasmid

#104001

Purpose

Plasmid encoding mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRFP1

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mTq2

Plasmid

#67031

Purpose

Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-iRFP

Plasmid

#175830

Purpose

Express iRFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mPapaya1-H2B-C-10

Plasmid

#56401

Purpose

Localization: Nucleus/Histones, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

H2B (H2BC11 Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-jGCaMP8f

Plasmid

#172387

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8f

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pX458_miR-290-295KO-gRNA1

Plasmid

#172709

Purpose

Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_miR-290-295KO-gRNA2

Plasmid

#172710

Purpose

Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-tdTomato

Plasmid

#172382

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + tdTomato

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-Sec61β

Plasmid

#174467

Purpose

Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-PDHA1-N-10

Plasmid

#55909

Purpose

Localization: Mitochondria, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PDHA1 (PDHA1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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BAS287 C terminal E2C hygBR

Plasmid

#74082

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB1-C-18

Plasmid

#55415

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB1 (MAPRE1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
Clover-Nup50-N-10

Plasmid

#56303

Purpose

Localization: Nuclear Pore, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Nup50 (NUP50 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
p148

Plasmid

#62616

Purpose

ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRQ-Id1HA

Plasmid

#18112

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

Inhibitor of differentiation 1 (Id1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-CHAD

Plasmid

#127793

Purpose

Expresses the CHAD domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF-CHAD (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 201-433)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Dectin1A-N-10

Plasmid

#55291

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Dectin1A (CLEC7A Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits