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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,447 results for: grn

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Showing: 10621 - 10640 of 15447 results

  2. pX459-sg-tdTomato90/816

    Plasmid
    #179918
    Purpose
    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.
    Depositor
    Indeever Madireddy, Johan Sosa
    Publication
    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato sgRNA
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179918-plasmid-map-sequence-id-358501

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  3. pGG426_UV5

    Plasmid
    #165608
    Purpose
    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1135V, S1136R, D1332K and R1333G mutations in Sp…
    Promoter
    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165608-plasmid-map-sequence-id-330965

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  4. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  12. sg1+sg2+sg3

    Plasmid
    #113969
    Purpose
    Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC
    Depositor
    Jordan Green
    Publication
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sg1+sg2+sg3
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    113969-plasmid-map-sequence-id-222998

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  13. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  14. pTFAP2C.1.0-gDNA

    Plasmid
    #113792
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2C
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TFAP2C (TFAP2C Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113792-plasmid-map-sequence-id-241260

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  15. pZNF423.1.0-gDNA

    Plasmid
    #113790
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF423
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF423 (ZNF423 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113790-plasmid-map-sequence-id-230593

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  19. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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Showing: 10621 - 10640 of 15447 results