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Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS12
Plasmid#139419PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.DepositorInsertSuperfolder GFP
ExpressionYeastPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
iRFP-N1-PI-PLCC100-FRB
Plasmid#139306PurposeC-terminal half of split PI-PLCDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertL. monocytogenes PI-PLC(188-287):IGTAGPRSANS[GA]4: MTOR(2021-2113):iRFP
ExpressionMammalianAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1
Plasmid#133340Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA_gcrA
UseCRISPRPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA
Plasmid#133334Purposefor constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA_ctrA
UseCRISPRPromoterconstitutiveAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
TagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1415 GFP-TDP43 VLIM-S
Plasmid#133323PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELF1_pet28a
Plasmid#131649PurposeBacterial expression of ETS gene ELF1DepositorAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHisExpressionBacterialMutationwith TEV site between RGG domainsAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-TagBFP
Plasmid#117792PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-eGFP
Plasmid#117794PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-TagBFP
Plasmid#117786PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSELt_S7.A
Plasmid#125835PurposeNegative selection plasmid for evolution of an amber stop codon suppression tRNADepositorInsertACP-2xAmb-pheS
UseSynthetic BiologyExpressionBacterialPromoterpr.trpL*Available sinceMay 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBS1084 GFP-TDP43 deltaCTD
Plasmid#118798PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NDP52(V136S/C443K)
Plasmid#119686PurposeFor mammalian expression of LIR/UBD double mutant NDP52DepositorAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only