We narrowed to 12,079 results for: ARIA;
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Plasmid#100839PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6m
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterCAGAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable SinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rescue Septin 11 mRNA
Plasmid#122022PurposeRescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
ExpressionMammalianMutationSilent mutations are in 5 consecutive codons (AUU…Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK8
Plasmid#122677PurposeExpresses mouse PCSK8 in mammalian cellsDepositorInsertMus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
ExpressionMammalianPromoterT7Available SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D2s-L-V1
Plasmid#19967DepositorInsertD2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)
TagsFlag and N terminal VenusExpressionMammalianAvailable SinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
ExpressionMammalianPromoterCAGAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
ExpressionWormMutationYFP with destabilized signal from mouse ornithine…Available SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
TagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…Available SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_altPKA-Cα-mEGFP
Plasmid#114132PurposeAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorInsert18747 (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA-NG
Plasmid#220896PurposeExpress haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-VA-NG
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee-Kras
Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
TagsFLAG and mRFPExpressionMammalianPromoterCAGAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV-Beta(4)Integrin
Plasmid#16265DepositorInsertBeta 4 Integrin (ITGB4 Human)
ExpressionMammalianMutationlacked the 70- and 7-amino acid splice variants a…Available SinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151
Plasmid#190133Purposeexpresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianPromoterCMVAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
TagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only