We narrowed to 6,721 results for: pes

1. pAM PAT-ProCPC GW

   Plasmid

   #79757

   Purpose

   binary DEST vector for plant expression

   Depositor

   Martin Hulskamp, Nico Dissmeyer

   Article

   Pesch et al Development. 2013 Aug;140(16):3456-67. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ProCPC

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2312

   Plasmid

   #142028

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETV3 (ETV3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNAs[bTub+Sxl].1046B

   Plasmid

   #112692

   Purpose

   express two gRNA targeting bTub & Sxl under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2313

   Plasmid

   #142404

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETV3 (ETV3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA4008

   Plasmid

   #70109

   Purpose

   mtDNA destruction/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   Herpes simplex virus UL12.5M185
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   N-terminal deletion of UL12.5

   Promoter

   CMV and RSV

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47732:FCP:NAT

   Plasmid

   #85984

   Purpose

   Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   NAT
   

   Use

   Golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   FCP

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM601-TRA-2::FLAG

   Plasmid

   #27129

   Depositor

   Ding Xue

   Article

   Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

   Insert

   TRA-2 FLAG (tra-2 Nematode)
   

   Use

   Tags

   3x FLAG

   Expression

   Worm

   Mutation

   Replaced TRA-2 stop codon with KpnI and EcoRI and…

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM614-TRA-2::GFP

   Plasmid

   #27128

   Depositor

   Ding Xue

   Article

   Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

   Insert

   TRA-2 GFP (tra-2 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   replaced stop codon with AgeI and EcoRI added GFP…

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDA313

   Plasmid

   #195214

   Purpose

   Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   sfGFP with tetO and a hairpin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVG97

    Plasmid

    #164849

    Purpose

    Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. smHA multi-frame tag

    Plasmid

    #128611

    Purpose

    a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

    Depositor

    Tim Stasevich

    Article

    Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

    Insert

    smHA MF tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-fugu SLC2A4 promoter

    Plasmid

    #65101

    Purpose

    Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

    Depositor

    Josep Planas

    Article

    Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

    Insert

    SLC2A4 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

14. 6xmyc_cort_UAS-P

    Plasmid

    #112991

    Purpose

    P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

    Insert

    cort (Cdc20) (cort Fly)
    

    Use

    P insertion vector

    Tags

    6X myc epitope

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115210

    Purpose

    Expresses AspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    AspCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115213

    Purpose

    Expresses RanCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    RanCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Expression

    Mutation

    Promoter

    FCP and U6

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-NRCH-PE2-BSD

    Plasmid

    #191101

    Purpose

    Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    NRCH-PE2-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

    Plasmid

    #105201

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-IL12-P2A-CCL21-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLN472 (FuGW-G8p-STE-1Pe)

    Plasmid

    #105190

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-STE-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Sumo3 PolQM1 deltains2

    Plasmid

    #78460

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2264-2315; Q2513R (see deposi…

    Promoter

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

24. Sumo3 PolQM1 deltains3

    Plasmid

    #78459

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2497-2529

    Promoter

    T7

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pCW57-GFP-miR-302cluster

    Plasmid

    #132549

    Purpose

    Doxycycline-inducible expression of human miR-302 cluster

    Depositor

    Tomáš Bárta

    Article

    Peskova et al Sci Rep. 2019 Oct 31;9(1):15759. doi: 10

    Insert

    human miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367
    

    Use

    Lentiviral; Doxycycline inducible, microrna expre…

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

    Plasmid

    #199447

    Purpose

    Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    dSaCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJM31

    Plasmid

    #52986

    Purpose

    A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Annexin V (ANXA5 Human)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.41

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJM483

    Plasmid

    #52987

    Purpose

    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJM656

    Plasmid

    #53086

    Purpose

    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge