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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,392 results for: CIT

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Showing: 1061 - 1080 of 4392 results

  3. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  9. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pYPQ210

    Plasmid
    #109329
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection
    Depositor
    Yiping Qi
    Article
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Expression
    Plant
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    109329-plasmid-map-sequence-id-260867

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  17. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  18. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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Showing: 1061 - 1080 of 4392 results