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We narrowed to 63,783 results for: cloning

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Showing: 1061 - 1080 of 63783 results

  2. PB_tre_Cas9

    Plasmid
    #126029
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible Cas9
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    humanized S. pyrogenes Cas9
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Promoter
    EF1 alpha and TRE
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126029-plasmid-map-sequence-id-246111

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  3. EasyClone-MarkerFree Vector Set

    Plasmid kit
    #1000000098
    Purpose
    Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Yeast Expression
    Cloning type
    USER
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid
    #71719
    Purpose
    Expressing a GFP-tagged degron in the soma of C. elegans
    Depositor
    Abby Dernburg
    Publication
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    Tags
    EmGFP
    Expression
    Worm
    Mutation
    minimal degron sequence (71-114aa) with start cod…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71719-plasmid-map-sequence-id-246098

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  14. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    Encodes anti-HA scFv (anti-HA frankenbody) fused with mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    129593-plasmid-map-sequence-id-254271

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  15. TFORF3550

    Plasmid
    #145026
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145026-plasmid-map-sequence-id-422981

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  16. pCMV-MMLVgag-3xNES-ABE8e-NG

    Plasmid
    #181754
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e-NG
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181754-plasmid-map-sequence-id-353937

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  18. PB_tre_dCas9_KRAB

    Plasmid
    #126030
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9 fusion with KRAB
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    EF1 alpha and TRE
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126030-plasmid-map-sequence-id-245793

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  19. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid
    #46913
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-p65AD-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    2xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46913-plasmid-map-sequence-id-66611

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  20. pLenti6-MK1-EHMT2-V5

    Plasmid
    #31113
    Depositor
    Bernard Futscher
    Insert
    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Stop codon mutated to Ala
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31113-plasmid-map-sequence-id-504129

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Showing: 1061 - 1080 of 63783 results