We narrowed to 59,357 results for: cloning

1. C234-E07: attB2r-HA-attB3

   Plasmid

   #162911

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   HA epitope tag
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C234-E06: attB2r-FLAG-attB3

   Plasmid

   #162910

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   FLAG epitope tag
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. C234-E05: attB2r-V5-attB3

   Plasmid

   #162909

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   V5 epitope tag
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

   Plasmid

   #137110

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   MBP-CS(TEV)-StrepTag-II-AI(TVMV)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_C2515

   Plasmid

   #91083

   Purpose

   Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   AtU6

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_C2518

   Plasmid

   #91087

   Purpose

   Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   TaU6

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMOD_C2616

   Plasmid

   #91090

   Purpose

   Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   At7SL

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD_C2517

   Plasmid

   #91086

   Purpose

   Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   TaU3

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMOD_C2520

   Plasmid

   #91089

   Purpose

   Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_C2516

    Plasmid

    #91084

    Purpose

    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR P4-P1R-V5-6xHis

    Plasmid

    #48346

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Mutation

    Contains a Kozak sequence and initiation codon up…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD_C2519

    Plasmid

    #91088

    Purpose

    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    OsU3

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB-DEST (JDW 1205)

    Plasmid

    #229821

    Purpose

    A PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-Neo-DEST (JDW 940)

    Plasmid

    #229825

    Purpose

    A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-phiC31o (JDW 453)

    Plasmid

    #229842

    Purpose

    A CMV-driven Phi31 recombinase.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    phiC31 codon optimized, PhiC31o
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E089 Hs.FGFR4

    Plasmid

    #70373

    Purpose

    Gateway ORF clone of human FGFR4 [NM_002011.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E233 Hs.RASAL1

    Plasmid

    #70517

    Purpose

    Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL1 (RASAL1 Human)
    

    Use

    Gateway entry clone

    Mutation

    R331H

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E033 Hs.DUSP1

    Plasmid

    #70317

    Purpose

    Gateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP1 (DUSP1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. R777-E143 Hs.NFKB1

    Plasmid

    #70427

    Purpose

    Gateway ORF clone of human NFKB1 [NM_003998.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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