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Silene_conica _PheRS_PlastidTP_pK7FWG2

Plasmid

#202660

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF18_TurboID_3xHA

Plasmid

#200017

Purpose

Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF18

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF30_TurboID_3xHA

Plasmid

#200018

Purpose

Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF30

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF31_TurboID_3xHA

Plasmid

#200019

Purpose

Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF31

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF66_TurboID_3xHA

Plasmid

#200020

Purpose

Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF66

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_3xHA_TurboID_ORF34

Plasmid

#200021

Purpose

Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF34

Tags

3xHA-TurboID

Expression

Mammalian

Promoter

CMV promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF29

Plasmid

#200028

Purpose

Lentiviral vector for expression of KSHV ORF29

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pNL2114 pARF19 GG0-GG1

Plasmid

#195909

Purpose

Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19 (ARF19 Mustard Weed)

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-U6-sgGFP

Plasmid

#190899

Purpose

AAV vector expressing sgGFP

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA

Use

AAV and CRISPR

Promoter

U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmGW182-RBD_F

Plasmid

#146290

Purpose

Bacterial Expression of DmGW182-RBD

Depositor

Elisa Izaurralde

Article

Eulalio et al Nucleic Acids Res. 2009 May;37(9):2974-8

Insert

DmGW182-RBD (gw Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-delRBD_E

Plasmid

#146229

Purpose

Insect Expression of DmGW182-delRBD

Depositor

Elisa Izaurralde

Article

Eulalio et al Nucleic Acids Res. 2009 May;37(9):2974-8

Insert

DmGW182-delRBD (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmNot4_813-836opt_AG

Plasmid

#148793

Purpose

Bacterial Expression of DmNot4_813-836opt

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4_813-836opt (CG31716 Synthetic)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmNot4_813-836_AF

Plasmid

#148694

Purpose

Bacterial Expression of DmNot4_813-836

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4_813-836 (CG31716 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3_X

Plasmid

#147944

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-F4A_X

Plasmid

#147946

Purpose

Mammalian Expression of HsNanos3-F4A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-F4A (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-W7E_X

Plasmid

#147947

Purpose

Mammalian Expression of HsNanos3-W7E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-W7E (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-Y10E_X

Plasmid

#147948

Purpose

Mammalian Expression of HsNanos3-Y10E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-Y10E (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5_267-1017-V5-SBP-MBP_V

Plasmid

#147762

Purpose

Mammalian Expression of HsSmg5_267-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5_267-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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