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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33,015 results for: REP

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Showing: 10801 - 10820 of 33015 results

  1. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  4. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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  5. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180423-plasmid-map-sequence-id-354264

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  6. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212993-plasmid-map-sequence-id-423196

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  7. T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

    Plasmid
    #193359
    Purpose
    self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2A61V is a highly cooperative point mutant of …
    Promoter
    T7
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193359-plasmid-map-sequence-id-379851

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  9. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A set of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit (#1000000061) for protein expression and secretion in Pichia pastoris.
    Depositor
    Volker Sieber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    MoClo
    Available Since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Restriction Enzyme And Ligation (REAL) TALEN assembly of TALE repeat arrays. Includes vector backbones for mammalian expression or in vitro transcription to produce TALE nuclease mRNAs.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    Plasmids for inducible, linkage-specific polyubiquitylation of a protein of interest for in vitro experiments.
    Depositor
    Helle Ulrich
    Application
    Gene Expression and Labeling
    Vector Type
    In Vitro Expression
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4

    Plasmid
    #193360
    Purpose
    VEE-CFP-S*K (self-replicating RNA vector expressing CFP + human super-SOX + KLF4 + PuroR) for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    CFP-e2a-SOX2-17-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    T7
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193360-plasmid-map-sequence-id-379850

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  16. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Construct Platinum TALEN vectors, with variable TALE repeats and non-RVD variations for expression in cultured cells, nematodes, sea urchins, ascidians, newts, frogs, mice, or rats.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Eukaryotic Expression, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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Showing: 10801 - 10820 of 33015 results