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Plasmid#213352PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNPFF2 (NPFFR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EDNRA-DuET
Plasmid#213232PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEDNRA (EDNRA Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR5-DuET
Plasmid#213222PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR5 (CXCR5 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis S198A
Plasmid#213605PurposeExpresses PLA2G15-6xHis S198A in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
Tags6xHisExpressionMammalianMutationChanged serine 198 to alaninePromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-GRAB_UCN1.0
Plasmid#208667PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
ExpressionMammalianPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mCherryEA
Plasmid#207868PurposeMitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmito-mCherryEA
Tags4xCox8 mitochondrial targetingExpressionMammalianPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaSpCas9-NLS-6xHis
Plasmid#207386PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698A, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
MmEos3.2
Plasmid#203754PurposeEncodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSrc15 n-term (SRC Human)
TagsmEos3.2ExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)
Plasmid#197943PurposeExpression of CoA-Snifit in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHalo-GFP-ecPanK
ExpressionMammalianMutationPanK=PanK-G41S, D127A,L277W,F252YAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-PHOX2B-MUT
Plasmid#194577PurposeMammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion ProteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHOX2B (PHOX2B Human)
TagsmEGFPExpressionMammalianMutationS207Afs*102Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mRuby-Rab8a
Plasmid#194337PurposeExpresses mRuby Rab8a in mammalian cells and zebrafish embryosDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab8 (RAB8A Canis lupus)
TagsmRubyExpressionMammalianPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB8F+12
Plasmid#190854PurposeFor mammalian expression of 3JB8F+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB8F_T1+12bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bassoon scFv [L124/59]
Plasmid#190508PurposeMammalian Expression of Bassoon scFV. Derived from hybridoma L124/59.DepositorInsertBassoon (Rattus norvegicus) recombinant scFV (Bsn Mouse)
TagsHA, Sortase, 6xHisExpressionMammalianPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc
Plasmid#186987PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, Gluc crRNA guide
UseAAVMutations1006-gggs-d1221 deletionAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-WT
Plasmid#182741PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase (ALOX5 Human)
UseCre/Lox and LentiviralExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-CD79B-YFP
Plasmid#187006PurposeExpression of human CD79B as YFP fusion proteinDepositorAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT527
Plasmid#180514PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ194
Plasmid#163640Purposerps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
UseCRISPRTags2xmKate2 and GFPExpressionWormPromoterrps-27Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only