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pEVOL-pylT-AznLRS

Plasmid

#172482

Purpose

For E.Coli expression, 306L309L348I384F411W

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

Insert

AznLRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJAT77

Plasmid

#204295

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-H2Kb2

Plasmid

#17852

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

H-2Kb (H2-K1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pMHG-W57* reporter

Plasmid

#72850

Purpose

GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.

Depositor

Daniel Liebler

Article

Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 20

Insert

GFP

Expression

Mammalian

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJUMP44-2A(sfGFP)

Plasmid

#126988

Purpose

Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE5

Plasmid

#90339

Purpose

STAT3 - Stat3 homodimer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT3

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE-IRES-Blast

Plasmid

#213115

Purpose

Expression of untagged MinE in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE (minE )

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Article

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proC-B0032-E0051

Plasmid

#107242

Purpose

Expresses lacZa-GFP fusion from constitutive proC promoter

Depositor

Joseph Davis, Robert Sauer

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProC-RBS(B0032)-Gemini (E0051)

Promoter

proC

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proB-B0032-E0051

Plasmid

#107243

Purpose

Expresses lacZa-GFP fusion from constitutive proB promoter

Depositor

Joseph Davis, Robert Sauer

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProB-RBS(B0032)-Gemini (E0051)

Promoter

proB

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pB-CuO-ZNF729-HA-CymR-Puro

Plasmid

#248164

Purpose

Expresses ZNF729 upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

ZNF729 (ZNF729 Human)
HA-CymR-T2A-puro

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1

Plasmid

#217486

Purpose

Expression of monomeric Enhanced Green Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry Tensin3

Plasmid

#105301

Purpose

To study localization and function of Tensin 3 with EGFP Tensin1

Depositor

Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin3 (TNS3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pwtCas9-bacteria

Plasmid

#44250

Purpose

aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

wild-type Cas9

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Dazl-CPG-TV

Plasmid

#70144

Purpose

a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL

Use

Mouse Targeting and Synthetic Biology

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Eps15-pmCherryN1

Plasmid

#27696

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Eps15 (EPS15 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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