We narrowed to 15,044 results for: invs

1. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAc5.1B-EGFP-DmThor-V5His6

   Plasmid

   #79408

   Purpose

   Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmThor (Thor Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP and V5-His6

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Opto-GPR19

   Plasmid

   #106007

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR19 (GPR19 Bovine, Human)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcdna3 Flag Jip3b(T266A, T276A, T287A)

   Plasmid

   #58924

   Purpose

   interacts with MAPK signaling module

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

   Insert

   mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed T266A, T276A, T287A

   Promoter

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pTBL681 CHYRON4 integration construct

   Plasmid

   #126449

   Purpose

   To integrate the CHYRON4 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON4 hgRNA
   pCMV-puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

   Plasmid

   #121800

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121819

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/Flag-hINO80N1 (1-262)

   Plasmid

   #29441

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 1-262 (a.a.)

   Promoter

   Available Since

   Aug. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-FLAG-hDIXDC1 S592A

   Plasmid

   #61223

   Purpose

   Mammalian expression vector encoding long isoform of human DIXDC1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   human DIXDC1 (DIXDC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Serine 592 to Alanine

   Promoter

   CMV

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Myc-Idax DBM

    Plasmid

    #68554

    Purpose

    This plasmid expresses full length IDAX containing a DNA binding mutation.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    IDAX-DBM (Cxxc4 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T162A, H164A and Q165A mutations

    Promoter

    EF1a

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRD439

    Plasmid

    #168465

    Purpose

    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mTurquoise2-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

    Plasmid

    #14442

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/HisMaxB-YAP2-1st WW mutant

    Plasmid

    #18998

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine, and Proline 2…

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2-Cx43-ML-HA

    Plasmid

    #49857

    Purpose

    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All internal Methionines mutated to Leucine. Cont…

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1mLCAD_K42R_FLAG

    Plasmid

    #24486

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Arg

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Opto-GPR75

    Plasmid

    #106032

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR75 (GPR75 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/HisMaxB-YAP2-2nd WW mutant

    Plasmid

    #18999

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 258 changed to Alanine, and Proline 2…

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcDNAM-human eIF2C1-WT-Myc

    Plasmid

    #50360

    Purpose

    Expression of human eIF2C1 in mamalian cells

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C1 (AGO1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Opto-GPR61

    Plasmid

    #106027

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR61 (GPR61 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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