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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Ecoli PancACe

Plasmid

#215712

Purpose

Expresses acetyl-CoA biosensor in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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mito-mGarnet2

Plasmid

#104310

Purpose

codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mito-mGarnet2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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AsdCas12a KRAB

Plasmid

#188502

Purpose

Expresses FLAG tagged AsdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mWasabi

Plasmid

#169409

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mWasabi

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

Plasmid

#125695

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mScarlet-Gephyrin.FingR-IL2RGTC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP718nano

Plasmid

#195744

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p.UTA.3.0 Empty

Plasmid

#82447

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hfYFP

Plasmid

#186523

Purpose

Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-SFNp-mRFP1

Plasmid

#238473

Purpose

Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

Depositor

Christopher Nelson

Article

Ivy et al BMC Methods. 2025;2(1):8. doi: 10.1186/s

Insert

SFNp-mRFP1

Promoter

SFNp

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Cyto-GA

Plasmid

#209859

Purpose

Dimerization dependent fluorescent protein GA fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP161

Plasmid

#189536

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-161-GFP

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Syn-PSD95.FingR-eGFP

Plasmid

#126212

Purpose

Labeling of excitatory synapses (green fluorescence) in dividing cells

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP

Use

Retroviral

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Slow-FT

Plasmid

#31915

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTRE-Fast-FT

Plasmid

#31913

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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Lyso-GA

Plasmid

#209874

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP1_r1c3

Plasmid

#65724

Purpose

Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-hfYFP

Plasmid

#186522

Purpose

Hyperfolder YFP fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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VAChT-pHuji

Plasmid

#105319

Purpose

VAChT tagged with pH-sensitive red fluorescent protein pHuji

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAChT (Slc18a3 Rat)

Tags

pHuji

Expression

Mammalian

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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VAMP2-pHuji

Plasmid

#105289

Purpose

VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2 (Vamp2 Rat)

Tags

pHuji

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-smURFP-RBS-HO-1

Plasmid

#80341

Purpose

Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-N1

Plasmid

#136558

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav-GA

Plasmid

#209880

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP713-N1

Plasmid

#136559

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-FRISZ

Plasmid

#176887

Purpose

Far-red fluorescent zinc indicator FRISZ in pDisplay

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-smURFP-T2A-mCardinal

Plasmid

#80348

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCardinal

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-Medium-FT

Plasmid

#31914

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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