We narrowed to 34,801 results for: Nov
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Plasmid#196678PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.DepositorInsertPKIαmut-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR
Plasmid#195532PurposeCre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_CEACAM6-His
Plasmid#149337Purposefor eukaryotic expression and purification of CEACAM6-His6DepositorInsertCEACAM6 (CEACAM6 Human)
UseTagsIL-2 signal peptide and polyhistidineExpressionMammalianMutationpoint mutation (GGA to GCA) to replace 'G-g…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-CFL-SN
Plasmid#181740PurposeAAV vector for expressing CFL-SNDepositorInsertCFL-SN
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
UseTagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
Plasmid#71282PurposeZebrafish expression vector for heat-shock inducible expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterHSP70lAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCherry-EF(C)
Plasmid#61664PurposeTandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linkerDepositorInsertKIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-OSBP
Plasmid#67664PurposeMammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-OSBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAce-Kv2.1PR
Plasmid#195523PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xFYVE_hrs
Plasmid#67663PurposeMammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-2xFYVE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2-Kv2.1PR
Plasmid#195528PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-H2BC11-mR2
Plasmid#183866PurposeRepair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.DepositorInsertH2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mRuby2ExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mR2-MYH10
Plasmid#183869PurposeRepair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.DepositorInsertMYH10 homology arms with mRuby2-linker (MYH10 Human)
UseCRISPR; Donor templateTagsmRuby2-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)-L-GFP
Plasmid#193716PurposeExpression of GFP tagged human kinesin-1 (KIF5B aa1-560)DepositorInserthsKIF5B(1-560)-L-GFP (KIF5B Synthetic, Human)
UseTagsEGFPExpressionMammalianMutationhsKIF5B(aa1-560);PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-RHOA_sgRNA
Plasmid#183878PurposepX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.DepositorInsertRHOA sgRNA spacer (RHOA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only