We narrowed to 14,414 results for: TIM;

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A

Plasmid

#241246

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) (BLTP3A Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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iSpyMac

Plasmid

#155014

Purpose

Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

Insert

iSpyMac

Expression

Mammalian

Mutation

PID grafted onto SpyCas9 N-terminal domain, R221K…

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDACR3882 [rab-3p::HYlight::let-858utr]

Plasmid

#207666

Purpose

Expresses worm-optimized HYlight in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight Biosensor

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-pth2

Plasmid

#191428

Purpose

Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

Depositor

Timothy Erickson

Article

Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

Insert

pth2 regulatory region (pth2 Zebrafish)

Use

Tol2 gateway 5' entry vector

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Has Service

AAV6 and AAV8

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

Plasmid

#168291

Purpose

optimized miniCMV (OminiCMV)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

mCherry

Expression

Mammalian

Promoter

miniCMV

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-P2A-GFP

Plasmid

#247161

Purpose

In vitro transcription of human codon optimized wildtype Dn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-BRD4ΔN

Plasmid

#200968

Purpose

induction of phase-separated condensate mediated by BRD4dN-IDR

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

BRD4dN

Expression

Bacterial and Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRi_Mxi1_yl

Plasmid

#91248

Purpose

CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

Inserts

Codon optimized dCas9-Mxi1
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5FRT/TO_HA-mAID-nanobody

Plasmid

#117713

Purpose

Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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