We narrowed to 59,942 results for: %s

1. pMSCV-puro-mMCL1 Delta C22

   Plasmid

   #45820

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   mMCL-1 Delta C22
   

   Expression

   Mammalian

   Mutation

   C-terminal 22 amino acids are deleted from full l…

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pMSCV-puro-Delta N67-mMCL1

   Plasmid

   #45819

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   Delta N67-mMCL-1
   

   Expression

   Mammalian

   Mutation

   N-terminus 67 amino acids deleted from full lengt…

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAV10.K3.loxP

   Plasmid

   #63202

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV10.K5.loxP

   Plasmid

   #63204

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV10.HO3.loxP

   Plasmid

   #63205

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV10.HO6

   Plasmid

   #63211

   Purpose

   pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYCTK034

   Plasmid

   #176738

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   bar1Sc
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYCTK027

   Plasmid

   #176731

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   ste2Sc
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVW296

    Plasmid

    #139496

    Purpose

    Expression vector for the PP7-mCherry protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 PP7∆FG-mCherry tCYC1
    

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH6HTC_TKT-Halo

    Plasmid

    #175331

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    TKT (TKT Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAH01Sp::Cas9D10A

    Plasmid

    #128161

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9D10A variant

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSP561

    Plasmid

    #139497

    Purpose

    Expression vector for the MS2-GFP protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 MS2-GFPenvy tCYC1
    

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKF295

    Plasmid

    #72871

    Purpose

    expression of improved FLP recombinase

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Expression

    Insect

    Mutation

    P2->S, added NLS

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAMS366-4xCDRE-GFP-PEST

    Plasmid

    #138658

    Purpose

    destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

    Depositor

    Sabrina Büttner

    Article

    Diessl et al Microbial Cell, 2020: in press

    Insert

    4xCDRE-CYC1(PM)-yEGFP3-PEST
    

    Use

    Yeast reporter plasmid

    Promoter

    CYC1

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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