We narrowed to 23,538 results for: Sis

Showing: 11061 - 11080 of 23538 results

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pAL190

Plasmid

#22279

Purpose

Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
Ad-CBR (GFP/cAPC)

Plasmid

#16576

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

cAPC (APC Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Residues 958-2076

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pAcGP67A-rCL1-HormR-GAIN

Plasmid

#37847

Depositor

Axel Brunger

Article

Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

Insert

CL1 (Adgrl1 Rat)

Use

Baculovirus transfer vectors

Tags

8xHIS

Mutation

HormR and GAIN domains only (aa460–849

Promoter

Polyhedrin

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

35S

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtTcR1AtR2NtB2

Plasmid

#160889

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-Tc

Expression

Bacterial

Promoter

rbcS-Tc: T7, chaperones: T12

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Article

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-EF1a-mTagBFP2

Plasmid

#160507

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXR1AtR2B2NtS1

Plasmid

#160879

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: pBAD, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB528

Plasmid

#74870

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562A

Plasmid

#191976

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562A (Mfn1 Mouse)

Expression

Mammalian

Mutation

T562A

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562D

Plasmid

#191977

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562D (Mfn1 Mouse)

Expression

Mammalian

Mutation

T562D

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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UKNP5.1.1

Plasmid

#98271

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP4.1.1

Plasmid

#98266

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0897

Plasmid

#177090

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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