168,004 results

1. Non tagged Avidin

   Plasmid

   #214835

   Purpose

   This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Non tagged Avidin
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-jAspSnFR3

   Plasmid

   #203488

   Purpose

   jAspSnFR3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-ChR2-TdTomato

   Plasmid

   #166600

   Purpose

   Encodes Cre-dependent ChR2-TdTomato under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   ChR2-TdTomato
   

   Use

   AAV

   Mutation

   H134R

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-hAgo2

   Plasmid

   #21981

   Depositor

   Phil Sharp

   Article

   Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

   Insert

   Argonaute 2 (AGO2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRY2PHR-mCherryN1

   Plasmid

   #26866

   Purpose

   Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2PHR (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMyC

   Plasmid

   #42192

   Depositor

   Matthias Wilmanns, Annabel Parret

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   ColE1

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. dg263_pENTR_ChrimsonCel

   Plasmid

   #66101

   Purpose

   Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

   Depositor

   Dominique Glauser

   Article

   Schild et al Genetics. 2015 May 28. pii: genetics.115

   Insert

   Chrimson
   

   Use

   Gateway

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP NLS Beta-Actin G13R

   Plasmid

   #60615

   Purpose

   Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   G13R

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55645

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV5, AAV8, and AAV9

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-3xFLAG-Blast

    Plasmid

    #207717

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mTFP1-pBAD

    Plasmid

    #54553

    Purpose

    Localization: Protein Expression Vector , Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

    Type

    Empty backbone

    Tags

    6xHis and mTFP1

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet S2

    Plasmid

    #171921

    Purpose

    Express RNA-based (p)ppGpp sensor and a NirFP

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Inserts

    RNA-based (p)ppGpp sensor
    NirFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Luciferase-pcw107-V5

    Plasmid

    #64649

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 Flag DLC1

    Plasmid

    #24265

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC1 (DYNLL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPOTv7-hygro-3HaloTag

    Plasmid

    #179828

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x HaloTag
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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