We narrowed to 59,246 results for: %s

1. Hygro-Cas9 donor

   Plasmid

   #86883

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

   Depositor

   Danwei Huangfu

   Article

   Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-HLA-A2

   Plasmid

   #135504

   Purpose

   Mammalian expression of myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-hMALAT1

   Plasmid

   #118580

   Purpose

   to overexpress human MALAT1 in mammalian cells

   Depositor

   Jianjun Zhao

   Article

   Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

   Insert

   MALAT1 (MALAT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-ZDHHC5

   Plasmid

   #231654

   Purpose

   Expresses human ZDHHC5 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC5 (ZDHHC5 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv2-STING_gRNA3

   Plasmid

   #127640

   Purpose

   Knock-out of human STING

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPEN-A*24:02

   Plasmid

   #235133

   Purpose

   E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. OPEN-HLA-A*01:01

   Plasmid

   #235134

   Purpose

   E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*01:01 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. OPEN-HLA-A*11:01-BirA

   Plasmid

   #235135

   Purpose

   E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*11:01 (HLA-A Human)
   

   Use

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 HA eIF4GI (1-1599)

    Plasmid

    #45640

    Depositor

    Nahum Sonenberg

    Article

    Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

    Insert

    eIF4GI transcript variant 2 (EIF4G1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PfV-Sapphire-IRES-DSRed

    Plasmid

    #116934

    Purpose

    Mammalian lenti vector for expression of the 40nm GEM and DSRed

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28A-Rab8A

    Plasmid

    #228880

    Purpose

    Expression of His-tagged Rab8A in E. coli

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    residues 1-6 and 177-207 missing

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HRD1

    Plasmid

    #231688

    Purpose

    Expresses human HRD1 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    HRD1 (SYVN1 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX5X.1 PINK1 WT

    Plasmid

    #13321

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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