We narrowed to 45,592 results for: cha;
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Plasmid#166725Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71102
Plasmid#154065PurposeLevel 0 Golden Gate vector; Unmodifed CREDepositorInsertCRE
UseSynthetic Biology; Standard cloning vector used b…MutationAll BsaI, Esp3I and BPiI restriction sites were r…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.2
Plasmid#149431PurposeminiTdg gene, SUMOylation mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxExpressionMammalianMutationK341A SUMOylation mutant, SNPs of Tdg gene in OLA…PromoterTdg promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.1
Plasmid#149430PurposeminiTdg gene, catalytic mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxExpressionMammalianMutationN151A catalytic mutant, SNPs of Tdg gene in OLA/1…PromoterTdg promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-delCry2
Plasmid#153545Purposethis is the non-clustering version of LIC-Z tagged with mcherryDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsmCherryExpressionMammalianMutationwith Cry2 sequence deleted from LIC-ZPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX30-PX (86-205)
Plasmid#119109PurposeBacterial expression of human phox homology (PX) domain, SNX30-PX (86-205)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-2
Plasmid#110365PurposeMammalian expression of HisA-tagged HOXC6-2DepositorAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
Tags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
Plasmid#126738Purposeyeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2,acs1*
Tags6xHis,MycExpressionYeastMutationT3542CPromoterGAPAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-9-G, 15-C
Plasmid#124688PurposeTuning expression of PD-1DepositorAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS K283D Q287D
Plasmid#79068PurposeExpress owl monkey TRIMCyp K283D, Q287D in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
TagsOneSTrEP-FLAGExpressionMammalianMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterCMVAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
TagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
TagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-K341R-IntE2
Plasmid#52269PurposeBacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertTDG (Tdg Mouse)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hoxd13-3'UTR
Plasmid#31525DepositorAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
SNX9-PX (246-376)
Plasmid#119090PurposeBacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)DepositorAvailabilityAcademic Institutions and Nonprofits only -
PI3KC2gamma-PX (1200-1310)
Plasmid#119121PurposeBacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)DepositorAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta 6X mutant
Plasmid#128816PurposeTo make virus expressing Doc2beta 6X mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPMutationD163A, D218A, D220N, D303A, D357A, D359APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2
Pooled Library#73179PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas ServiceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only