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pGMC00007

Plasmid

#166725

Purpose

non targeting sgRNA

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

Non-targeting guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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EC71102

Plasmid

#154065

Purpose

Level 0 Golden Gate vector; Unmodifed CRE

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE

Use

Synthetic Biology; Standard cloning vector used b…

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.1

Plasmid

#149430

Purpose

miniTdg gene, catalytic mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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SNX30-PX (86-205)

Plasmid

#119109

Purpose

Bacterial expression of human phox homology (PX) domain, SNX30-PX (86-205)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX30-PX (86-205) (SNX30 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScACS1*

Plasmid

#126736

Purpose

yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1*

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, P113S, and S629N (please see dep…

Promoter

GAP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

Plasmid

#126738

Purpose

yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2,acs1*

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C

Promoter

GAP

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-9-G, 15-C

Plasmid

#124688

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

Plasmid

#79068

Purpose

Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET-20b-T7-Ntx-K49

Plasmid

#58715

Purpose

Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Changed Aspartic Acid 49 to Lysine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-mTDG-K341R-IntE2

Plasmid

#52269

Purpose

Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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Hoxd13-3'UTR

Plasmid

#31525

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxd13-3'UTR (Hoxd13 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
SNX9-PX (246-376)

Plasmid

#119090

Purpose

Bacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX9-PX (246-376) (SNX9 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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PI3KC2gamma-PX (1200-1310)

Plasmid

#119121

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2gamma-PX (1200-1310) (PIK3C2G Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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Human sgRNA library Brunello in lentiCRISPRv2

Pooled Library

#73179

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only