We narrowed to 1,827 results for: PORA

1. FKBP-alpha(740-977)-GFP

   Plasmid

   #100731

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2A1 (Ap2a1 Mouse)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEX-Antenna-GDI RhoA

   Plasmid

   #189669

   Purpose

   Expression of Antenna-RhoA

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LightR-iCre-miRFP670

   Plasmid

   #162158

   Purpose

   Expresses LightR-iCre-miRFP670 in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-iCre-miRFP670
   

   Use

   Tags

   NLS and miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEX-Antenna-GDI Cdc42

   Plasmid

   #101681

   Purpose

   "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Cdc42
   

   Use

   Tags

   6xHis and FRET Antenna (mCerulean-cpVenus)

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mGrn-myc

   Plasmid

   #178944

   Purpose

   Construct for expressing murine progranulin (Grn) in AAV

   Depositor

   Erik Roberson

   Article

   Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

   Insert

   Grn (Grn Mouse)
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXY349

   Plasmid

   #200262

   Purpose

   Expressing FtsW-TagRFP-T in bacterial cells

   Depositor

   Jie Xiao

   Article

   Yang et al Nat Microbiol. 2021 May;6(5):584-593. do

   Insert

   FtsW-tagRFP (ftsW )
   

   Use

   Tags

   tagRFP

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6, EF-1a

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA1 Flag ATXN1L

   Plasmid

   #33242

   Depositor

   Huda Zoghbi

   Article

   Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

   Insert

   ATXN1L (ATXN1L Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89870

    Purpose

    expresses DRD2-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    bfp

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. XW17

    Plasmid

    #65835

    Purpose

    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL7.0-iRFP670-micro

    Plasmid

    #135954

    Purpose

    Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    iLID micro (SspB R73Q)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Use

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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