We narrowed to 23,538 results for: Sis

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

Plasmid

#67932

Purpose

Translational reporter - Lox 5'UTR

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry (LOX Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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cycLuc_SuMMVs

Plasmid

#119210

Purpose

Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with SuMMVp cleavage site

Use

Luciferase

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLsCas13aGG

Plasmid

#131012

Purpose

Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of LsCas13a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-Dusp6DM_S159/197A

Plasmid

#67684

Purpose

3' entry clone with activated Dusp6 (S159/167A)

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Mutation

S159A, S197A

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB525

Plasmid

#74867

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A

Plasmid

#32214

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala

Promoter

CMV

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_VAV3-1/2

Plasmid

#91528

Purpose

Protein expression and purification of human SH3 domain construct VAV3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV3-1/2 (VAV3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS3-1/3

Plasmid

#91534

Purpose

Protein expression and purification of human SH3 domain construct SORBS3-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS3-1/3 (SORBS3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV3-2/2

Plasmid

#91524

Purpose

Protein expression and purification of human SH3 domain construct VAV3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV3-2/2 (VAV3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3YL1-1/1

Plasmid

#91485

Purpose

Protein expression and purification of human SH3 domain construct SH3YL1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3YL1-1/1 (SH3YL1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEBL-1/1

Plasmid

#91383

Purpose

Protein expression and purification of human SH3 domain construct NEBL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEBL-1/1 (NEBL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NPHP1-1/1

Plasmid

#91358

Purpose

Protein expression and purification of human SH3 domain construct NPHP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NPHP1-1/1 (NPHP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN1-1/1

Plasmid

#91372

Purpose

Protein expression and purification of human SH3 domain construct PACSIN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN1-1/1 (PACSIN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D21-1/3

Plasmid

#91296

Purpose

Protein expression and purification of human SH3 domain construct SH3D21-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D21-1/3 (SH3D21 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO15A-2/2

Plasmid

#91291

Purpose

Protein expression and purification of human SH3 domain construct MYO15A-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO15A-2/2 (MYO15A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEYFP-Pds1Δdestruction box(383)

Plasmid

#39848

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

Pds1 (PTTG1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

Δdestruction box = ΔR61-N68

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pSYN-VWF-039

Plasmid

#120292

Purpose

VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

Depositor

Robert Peters

Article

Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

Insert

VWF D1-D2-D'-D3-Fc

Tags

Fc

Expression

Mammalian

Mutation

C1099A/C1142A

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AA)

Plasmid

#24240

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pGWB429

Plasmid

#74823

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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