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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv 2024.11.01.621418

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW100002

Plasmid

#222440

Purpose

Level 0, CDS module (AATG-GCTT) containing rice OsL5.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

OsL5 (Oryza sativa)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn

Plasmid

#183577

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

Tet-On

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGST-RXRalpha

Plasmid

#16547

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

RXR alpha (RXRA Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-224

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP8m

Plasmid

#18717

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

FLAG-SENP8m (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C153A

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
PGK CFP

Plasmid

#200429

Purpose

Mammalian expression of CFP

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

CFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-CRKL-Y198FY207F

Plasmid

#32506

Depositor

John Groffen, Nora Heisterkamp

Article

de Jong et al Oncogene. 1997 Feb 6;14(5):507-13.

Insert

CRKL (CRKL Human)

Expression

Mammalian

Mutation

Y207F and Y198F

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pTn7xTS-sfGFP

Plasmid

#117390

Purpose

Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO mTagBFP2

Plasmid

#68413

Purpose

TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTagBFP2

Use

Gmap

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLDPuro-MmnSR100N

Plasmid

#35171

Depositor

Benjamin Blencowe

Article

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

Srrm4 (Srrm4 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pJUMP44-2A(sfGFP)

Plasmid

#126988

Purpose

Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJAT56

Plasmid

#204300

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EGFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pN1-QNA

Plasmid

#129309

Purpose

Negative control for ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Xenopus C-cadherin

Plasmid

#17023

Depositor

Barry Gumbiner

Article

Lee et al Dev Biol. 1995 Oct . 171(2):363-73.

Insert

C-cadherin (cdh3.S Frog)

Use

Xenopus expression

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100A(K297A)-C1

Plasmid

#134552

Purpose

Expresses YFP-Sp100A fusion protein with a K297A mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100A(K297A)

Tags

YFP

Expression

Mammalian

Mutation

K297R point mutation in the SUMO site

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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