We narrowed to 18,398 results for: Mut

1. pYPQ159

   Plasmid

   #69308

   Purpose

   Gateway entry vector with hSpCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9D10A (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   D10A mutation; NLS fusion

   Promoter

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK371

   Plasmid

   #73596

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 3)
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Point mutants in FAD2-3 ORF relative to deposited…

   Promoter

   GAL1 and GAL10

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA HA FAK Y576F

   Plasmid

   #50506

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y576F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pUC19-U6-crRNA1

   Plasmid

   #123639

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   crRNA scaffold 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL006

   Plasmid

   #92329

   Purpose

   Express mEos3.2 tagged FtsZD212A mutant in E coli

   Depositor

   Jie Xiao

   Article

   Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

   Insert

   FtsZ
   

   Use

   Tags

   mEos3.2

   Expression

   Bacterial

   Mutation

   D212A

   Promoter

   T5-lac

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ptrc::RpaA D53A

   Plasmid

   #105229

   Purpose

   inducible expression of unphosphorylatable variant of RpaA (D53A)

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   rpaA driven by Ptrc promoter
   

   Use

   Cyanobacteria cloning vector

   Tags

   Expression

   Mutation

   D53 mutated to A

   Promoter

   ptrc

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEL2003

   Plasmid

   #139888

   Purpose

   Expression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta515-746)-6xHis

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEL2000

   Plasmid

   #139885

   Purpose

   Expression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta161-747)-6xHis

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH809_pJH_LldR_Pcon-sfGFP.10TAG

   Plasmid

   #163739

   Purpose

   Constitutive expression of green fluorescent protein encoding ten stop codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.10TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with first 10 leucine codons mutated to amber

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUMP27ts-1A(lacZ)

    Plasmid

    #126977

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH45244

    Plasmid

    #50277

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter + 5' UTR, RbcS3B (AT5g38410, A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-U6-BssgRNA

    Plasmid

    #121957

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-AmsgRNA

    Plasmid

    #121956

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-MAVS-M1A-invitro(KaganE12)

    Plasmid

    #52016

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-MAVS-G67M-invitro(KaganD100)

    Plasmid

    #52014

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Gly 67 to Met

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-MAVS-L62M-invitro(KaganD98)

    Plasmid

    #52013

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Leu 62 to Met

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-MAVS-E80M-invitro(KaganD99)

    Plasmid

    #52015

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Glu 80 to Met

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-MAVS-M142A-invitro(E13)

    Plasmid

    #52017

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M142A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 142 to Ala

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pfMDH_H303A

    Plasmid

    #67730

    Purpose

    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only