We narrowed to 172,922 results for: Gene

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pTarget TMEM97-P2A-mCardinal

Plasmid

#157747

Purpose

Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS_L_EBOV

Plasmid

#103052

Purpose

Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

L gene of Ebola virus (L Zaire ebolavirus, Mayinga)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEE844

Plasmid

#231167

Purpose

T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Type

Empty backbone

Use

Cloning vector

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-mFGF2

Plasmid

#219552

Purpose

mFGF2 expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

mFGF2 (Fgf2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMIEG3-c-Jun

Plasmid

#40348

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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IY601: pMVP/Lenti/Blast-DEST

Plasmid

#121849

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCHW101008

Plasmid

#222453

Purpose

Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::OsL5::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBHM2403

Plasmid

#171687

Purpose

pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

CnoCAS9

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

Plasmid

#110560

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

stRBS-lacZ

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)

Plasmid

#188379

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1

Plasmid

#119007

Purpose

Mammalian expression of human BRCA1 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Plasmid

#205162

Purpose

express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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BoxB in pcDNA3

Plasmid

#29727

Depositor

Howard Chang

Article

Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

Insert

5BoxB

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pQmod2S-GG

Plasmid

#191346

Purpose

Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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