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Plasmid#68616PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCDNA3 Flag Jnk2b1
Plasmid#13756DepositorInsertJnk2b1 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shABCB7_2
Plasmid#102969PurposeSuppression of ABCB7DepositorInsertshABCB7_2 (ABCB7 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shABCB7_1
Plasmid#102968PurposeSuppression of ABCB7DepositorInsertshABCB7_1 (ABCB7 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQsupR-Mi2b
Plasmid#15379DepositorInsertshort hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC12)_Psyn-sgRNArodA
Plasmid#149661Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC12), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS:bloSwitch
Plasmid#108355PurposeUAS driven green to red switch reporter for B3 recombinase expressionDepositorInsertUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
UseZebrafish expressionTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G1
Plasmid#188963PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: atcggtcgcattgttttccactagg
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Pdha1
Plasmid#175936Purposeknockout mouse Pdha1DepositorInsertPdha1 gRNA (Pdha1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nxph4-6A-HA
Plasmid#128103PurposeExpress HA tagged Nxph4-6ADepositorInsertNxph4 (Nxph4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Ld
Plasmid#135519PurposeMammalian expression of myc-tagged H2-LdDepositorInsertH2-Ld
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-FOP
Plasmid#136796PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN E50K
Plasmid#175765PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN E50KDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationE50KPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 SKICH (1-140D)
Plasmid#175768PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationSKICH (1-140D)PromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAPRE1
Plasmid#31589DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G20-R20
Plasmid#101822PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_WT
Plasmid#190743PurposeFor expression of wild-type rat SERT in mammalian cellsDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hRREB1-RFP
Plasmid#22924DepositorInserthuman ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-ARF1b
Plasmid#67494PurposeExpression of shRNA targeting human ARF1bDepositorInsertHsARF1 shRNA (ARF1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAftsI
Plasmid#149590Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
KK402: pMVP/Zeo-DEST
Plasmid#121859PurposepMVP destination vector, empty expression vector backbone w/ Zeo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP(K10)
Plasmid#121908PurposeExpresses BirA and eCPX-AP(K10)DepositorInsertsUseTags6xHisExpressionBacterialMutationchanged lysine 10 in the AP to alaninePromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-FOP
Plasmid#136797PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB314
Plasmid#68642PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S513A
Plasmid#175764PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS513APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GTF2F2
Plasmid#31323DepositorInsertgeneral transcription factor IIF (GTF2F2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v3
Plasmid#79539PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 3DepositorInsertAmrose v3
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB231
Plasmid#68622PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-KCNIP2
Plasmid#34799DepositorInsertKv channel interacting protein 2 (KCNIP2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-eGFP-SV40pA-FKF
Plasmid#119866PurposeDonor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB117
Plasmid#68571PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
Plasmid#149594Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-ATG101
Plasmid#171414PurposeFor the purifcation of human ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b
Plasmid#52122Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-ARF4a
Plasmid#67497PurposeExpression of shRNA targeting human ARF4aDepositorInsertHsARF4 shRNA (ARF4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-NOHMA
Plasmid#34821DepositorInsertHORMA domain containing protein (HORMAD1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only