We narrowed to 21,207 results for: Kin

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pcDNA3-HA-REDD1 77-232 (#426)

Plasmid

#99921

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 77-232

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-CCHD (p130Cas)

Plasmid

#145133

Purpose

Expresses CCH domain from p130Cas with an mCherry tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-SUB1

Plasmid

#178440

Purpose

doxycycline-inducible expression of Human SUB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SUB1 (SUB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-L166P/K130R

Plasmid

#29349

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Leu166Pro and Lys130Arg

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
MAC_N_ELK3

Plasmid

#167750

Purpose

Expresses ELK3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELK3 (ELK3 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/GS-DJ1-L166P-V5

Plasmid

#29343

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

L166P

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only
pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-FLAG-NEK9T210A

Plasmid

#168270

Purpose

Expresses kinase-dead NEK9 mutant in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed threonine 210 to alanine

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-p38 (Mapk14 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro-luc (shRNA)

Plasmid

#153962

Purpose

Expresses a shRNA against firefly luciferase

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

shRNA against firefly luciferase (19-bp-long target)

Use

Luciferase, RNAi, and Retroviral

Tags

N/A

Expression

Mammalian

Promoter

U6 promoter

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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3578 pcDNA3 Bad S112A

Plasmid

#8797

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S112A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal4 PGC1 alpha 1-350

Plasmid

#8893

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-350 of PGC-1 alpha

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-eYFP-3x-miR204-5p-TS

Plasmid

#117380

Purpose

An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCDF1-p18INK4c

Plasmid

#25650

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Apr 15. 68(8):2564-9.

Insert

p18INK4c (CDKN2C Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only