We narrowed to 23,077 results for: REP

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tetO-FUW-eGFP-RHOA-T19N

Plasmid

#73082

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-T19N

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

RHOA-T19N (RHOA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1285

Plasmid

#61252

Purpose

CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting pha-1

Use

CRISPR

Expression

Worm

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

Plasmid

#135955

Purpose

Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) N-terminal

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-Tre3G-47xCAG-12xMS2

Plasmid

#99148

Purpose

Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

47xCAG 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-Tre3G-29xGGGGCC-12xMS2

Plasmid

#99149

Purpose

Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

29xGGGGCC 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Article

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Article

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-WT

Plasmid

#25080

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pSVL(D3)

Plasmid

#29335

Depositor

John Taylor

Article

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Insert

Trimer of the HDV genome

Expression

Mammalian

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pMCS.DT-A

Plasmid

#22730

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

DT-A

Use

Bac recombineering

Mutation

This is a gap-repair vector designed for retrievi…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAd nGFP

Plasmid

#19413

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

nuclear GFP

Use

Adenoviral and Gateway Cloning

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

Plasmid

#69909

Purpose

Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pLSLGFP.R

Plasmid

#51501

Purpose

Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

GFP

Use

Plant t-dna plasmid

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET-PE2-His (IK1822)

Plasmid

#170103

Purpose

T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

Depositor

Keith Joung

Article

Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis

Use

CRISPR

Tags

6xHis tag

Expression

Bacterial

Mutation

Bacteria codon-optimized nSpCas9 (H840A) & M-…

Promoter

T7

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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