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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY140

Plasmid

#223487

Purpose

trp-4 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

trp-4 (trp-4 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY147

Plasmid

#223494

Purpose

unc-86 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-86 (unc-86 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY115

Plasmid

#223462

Purpose

pdfr-1 (3-2K) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

pdfr-1(3-2K) (pdfr-1 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY145

Plasmid

#223492

Purpose

unc-42 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-42 (unc-42 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY141

Plasmid

#223488

Purpose

trp-4(intron 1) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

trp-4(intron 1) (trp-4 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY146

Plasmid

#223493

Purpose

unc-46 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-46 (unc-46 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY119

Plasmid

#223466

Purpose

ser-2(promCE) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ser-2(promCE) (ser-2 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY143

Plasmid

#223490

Purpose

unc-31 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-31 (unc-31 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY112

Plasmid

#223459

Purpose

oig-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

oig-1 (oig-1 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY113

Plasmid

#223460

Purpose

osm-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

osm-10 (osm-10 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY133

Plasmid

#223480

Purpose

srz-45 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srz-45 (srz-45 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY130

Plasmid

#223477

Purpose

srx-113 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srx-113 (srx-113 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY131

Plasmid

#223478

Purpose

srx-105 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srx-105 (srx-105 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY137

Plasmid

#223484

Purpose

tax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tax-2 (tax-2 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY138

Plasmid

#223485

Purpose

tol-1(3k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tol-1(3k) (tol-1 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEY144

Plasmid

#223491

Purpose

unc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-39 (3k) (unc-39 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV cleavable His10

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-NeoR-SV40 polyA-Z

Plasmid

#218909

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-Neomycine-SV40 polyA

Expression

Mammalian

Mutation

395T>C

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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GB3668

Plasmid

#215243

Purpose

TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:mAID:AcrIIA4:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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