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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,499 results for: grna

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Showing: 11621 - 11640 of 16499 results

  3. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  10. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

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  13. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164423-plasmid-map-sequence-id-338872

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  18. BII-Sh-FUCCI

    Plasmid
    #133363
    Purpose
    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    Clover-Geminin-IRES-mKO2-Cdt1
    Tags
    HA-tagged Clover-Geminin
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133363-plasmid-map-sequence-id-267621

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Showing: 11621 - 11640 of 16499 results