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AL05_pDonor.DMD

Plasmid

#100284

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AC62_pDonor.DMD.TS.DR

Plasmid

#100288

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter 402 mutation

Plasmid

#89825

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Mutation

-370 Smad binding element mutated from CAGAC to T…

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

Plasmid

#58414

Purpose

Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA internal

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine; The sequenc…

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
Clover-MAPTau-C-10

Plasmid

#56371

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MAPTau (MAPT Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSBbi-GP-TFIIB(D207A)-2xStrep

Plasmid

#213920

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(D207A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(D207A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) D207A (2) Coding region changed for resistanc…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP-TFIIB(S65A)-2xStrep

Plasmid

#213921

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65A (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP-TFIIB(S65E)-2xStrep

Plasmid

#213922

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65E)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65E)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65E (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)

Plasmid

#254463

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP). Co-expression of PiggyBac transposase required. Tet-On 3G and green fluorescent protein mGreenLantern are co-expressed via the CAG promoter.

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)

Expression

Mammalian

Promoter

TRE3G

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAT159_AAV_p3_PE7-C_hU6_epegRNA

Plasmid

#251105

Purpose

Encodes the C terminal of the PE7 prime editor in an AAV expression plasmid + pegRNA cloning casette

Depositor

Gerald Schwank

Article

Talas et al Sci Transl Med. 2026 May 13;18(849):eaec

Insert

PE7 C-terminal

Use

AAV and CRISPR

Promoter

p3

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAT172_AAV_p3_PE7-N

Plasmid

#251106

Purpose

Encodes the N terminal of the PE7 prime editor in an AAV expression plasmid

Depositor

Gerald Schwank

Article

Talas et al Sci Transl Med. 2026 May 13;18(849):eaec

Insert

PE7 C-terminal

Use

AAV and CRISPR

Promoter

p3

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgPTPN11-1

Plasmid

#253090

Purpose

CRISPRi single-guide against PTPN11

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PTPN11 (PTPN11 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-ΔSH3-ΔM/F2

Plasmid

#251427

Purpose

Mammalian expression vector for expressing HaloTag-fused mouse MYO7A truncated after the first MyTH4-FERM domain

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (SH3 and M/F2 trancated) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

The SH3 and M/F2 domains trancated

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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Mito4x-LA-GCaMP6s

Plasmid

#243810

Purpose

Expresses a low affinity (LA) calcium sensor directed to the mitochondrial matrix

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Mito4x-LAGCaMP6s

Expression

Mammalian

Mutation

D362I

Promoter

CMV

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-ZNF207-3xFLAG

Plasmid

#231939

Purpose

Vector for generating stable lines allowing expression of ZNF207-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Insert

ZNF207 (ZNF207 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS1

Plasmid

#239460

Purpose

For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci Adv. 2025 Aug 29;11(35):eadw9153. do

Type

Empty backbone

Tags

VENUS:3xHA:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1385

Plasmid

#143105

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TMF1 (TMF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF3145

Plasmid

#144609

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF660 (ZNF660 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-UL36USP (C40)

Plasmid

#235548

Purpose

To express GFP-FLAG-UL36USP (C40) - region 25-520 of UL36

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

UL36USP (C40)

Use

Lentiviral

Tags

EGFP-FLAG

Mutation

N-terminal part of UL36 starting at the second AT…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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