We narrowed to 1,483 results for: Mbp

1. pEPQD0CM0545

   Plasmid

   #162296

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   codon optimized for E. coli

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQD0CM0544

   Plasmid

   #162295

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, MBP-[Factor Xa cleavage site]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   codon optimized for E. coli

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TbSIR2RP1

   Plasmid

   #67242

   Purpose

   Determine its activity and specificity

   Depositor

   Cynthia Wolberger

   Article

   Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

   Insert

   TbSIR2RP1
   

   Use

   Tags

   MBP, TEV, Thrombin, HIS, and none

   Expression

   Bacterial

   Mutation

   Promoter

   p-lac

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCri-1a-Strep

   Plasmid

   #61337

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and MBP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLIC.B4.BSU37660

   Plasmid

   #11535

   Depositor

   Sung-Hou Kim

   Article

   Xu et al J Struct Funct Genomics. 2005 Nov 9. ():

   Insert

   phosphotransacetylase
   

   Use

   Tags

   HisTag-MBP, TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

6. Sybody Generation Toolbox

   Plasmid Kit

   #1000000160

   Purpose

   The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

   Depositor

   Markus Seeger

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pRK793

   Plasmid

   #8827

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219V mutant
   

   Use

   Tags

   His, MBP (with TEV), and polyarginine

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability)

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSL103-SpCas9-FLAG-3XNLS

   Plasmid

   #182032

   Purpose

   Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

   Depositor

   Steven Lin

   Article

   Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

   Insert

   Streptococcus pyogenes cas9 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   12X His tag, 3XNLS, FLAG, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Use

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ubiquitin K48C

    Plasmid

    #86588

    Purpose

    Bacterial expression of Ubiquitin K48C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K48C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Ubiquitin K63C

    Plasmid

    #86589

    Purpose

    Bacterial expression of Ubiquitin K63C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K63C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMJ823

    Plasmid

    #39313

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (lin2744 Listeria innocua)
    

    Use

    CRISPR

    Tags

    MBP and thioredoxin-His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pHIT179

    Plasmid

    #204510

    Purpose

    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

    Depositor

    Yuji Kohara

    Article

    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

    Insert

    csr-1a (C. elegans) (csr-1 Nematode)
    

    Use

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMTTH+mEGFP-LANA

    Plasmid

    #157801

    Purpose

    Expression of mEGFP and LANA peptide fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dhh1-EGFP

    Plasmid

    #201418

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL-c2x-MT3

    Plasmid

    #32115

    Depositor

    Chris Mercogliano, Cristina Risco Ortiz, David DeRosier

    Article

    Diestra et al J Struct Biol. 2009 Mar;165(3):157-68. E

    Insert

    MT (3x) (Mt1 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    P-lac

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge