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Showing: 251 - 300 of 471 results

  2. pDA186

    Plasmid
    #74250
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pGPD1
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74250-plasmid-map-sequence-id-139759

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  3. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

    Plasmid
    #52898
    Purpose
    YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Ypd*1-L-IFP-F[2]
    Use
    Tags
    L-IFP-F[2]
    Expression
    Yeast
    Mutation
    Changed Tryptophane 80 to Alanine
    Promoter
    GAL1
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pDONR221-SLC4A11_STOP

    Plasmid
    #161358
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC4A11 (SLC4A11 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161358-plasmid-map-sequence-id-354482

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  18. pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156543
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1 (PDCD1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156543-plasmid-map-sequence-id-368511

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  22. pJL1050

    Plasmid
    #78186
    Purpose
    mCherry reporter expression in worms
    Depositor
    Junho Lee
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pIVTR(T7A)-SOX9

    Plasmid
    #172302
    Purpose
    In vitro transcription of SOX9
    Depositor
    Patrik Verstreken
    Insert
    SOX9 (SOX9 Human)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Jan. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    172302-plasmid-map-sequence-id-351374

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  24. pPD301 ZF43x16-Compact_EYFP

    Plasmid
    #138911
    Purpose
    EYFP reporter vector for the ZF43x16-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x16-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x16-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138911-plasmid-map-sequence-id-324231

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  25. pPD299 ZF43x14-Compact_EYFP

    Plasmid
    #138909
    Purpose
    EYFP reporter vector for the ZF43x14-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x14-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x14-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138909-plasmid-map-sequence-id-324222

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  26. pPD294 ZF43x10-Compact_EYFP

    Plasmid
    #138905
    Purpose
    EYFP reporter vector for the ZF43x10-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x10-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x10-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138905-plasmid-map-sequence-id-324140

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  27. pPD300 ZF43x15-Compact_EYFP

    Plasmid
    #138910
    Purpose
    EYFP reporter vector for the ZF43x15-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x15-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x15-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138910-plasmid-map-sequence-id-324228

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  28. pPD298 ZF43x13-Compact_EYFP

    Plasmid
    #138908
    Purpose
    EYFP reporter vector for the ZF43x13-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x13-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x13-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138908-plasmid-map-sequence-id-324143

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  29. pPD295 ZF43x11-Compact_EYFP

    Plasmid
    #138906
    Purpose
    EYFP reporter vector for the ZF43x11-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x11-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x11-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138906-plasmid-map-sequence-id-324141

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  30. pPD293 ZF43x9-Compact_EYFP

    Plasmid
    #138904
    Purpose
    EYFP reporter vector for the ZF43x9-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x9-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x9-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138904-plasmid-map-sequence-id-324139

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  31. pKPY738

    Plasmid
    #72674
    Purpose
    C. elegans let-858 3' UTR (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72674-plasmid-map-sequence-id-133995

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  32. pUPD2:SF35

    Plasmid
    #106216
    Purpose
    Provides short stuffer fragment of 35bp as a level 0 GoldenBraid part
    Depositor
    Tomáš Moravec
    Insert
    short stuffer fragment of 35bp
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106216-plasmid-map-sequence-id-205274

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  34. 8061-E01

    Plasmid
    #234239
    Purpose
    Gateway ORF Entry clone of human TGFB1 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    TGFB1 (TGFB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234239-plasmid-map-sequence-id-469709

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  35. pUPD2b:TMV-3NTR

    Plasmid
    #106218
    Purpose
    Provides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)
    Depositor
    Tomáš Moravec
    Insert
    Infectious clone of Tobacco mosaic virus, 3' part
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106218-plasmid-map-sequence-id-208888

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  37. hGli1 6x his

    Plasmid
    #84923
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  43. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

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  44. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

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  45. mEmerald-FilaminA-N-9

    Plasmid
    #54098
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. 8522-E01

    Plasmid
    #234287
    Purpose
    Gateway ORF Entry clone of human TGFB1 with stop codon (for native or N-terminal fusions); C223S/C225S mutations
    Depositor
    Dominic Esposito
    Insert
    TGFB1 C223S/C225S (TGFB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    C223S/C225S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234287-plasmid-map-sequence-id-469766

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  49. mCherry-FilaminA-N-9

    Plasmid
    #55047
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 251 - 300 of 471 results