We narrowed to 22,705 results for: ESC;

1. pDY1243-Fluorescent RADARS

   Plasmid

   #182540

   Purpose

   Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   mNeon
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-leu2d(empty)

   Plasmid

   #20120

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Expression

   Bacterial

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIplac128-pOst1-ESCargo

   Plasmid

   #115421

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Expression

   Yeast

   Promoter

   TPI1

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MH6-EsCas13d

   Plasmid

   #108303

   Purpose

   Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   EsCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GPX8 rescue PLAK1

   Plasmid

   #161517

   Purpose

   Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   glutathione peroxidase 8 (GPX8 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBluescript-mouse cyclin D2

   Plasmid

   #86791

   Purpose

   bacterial expression of mouse cyclin D2

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D2 (Ccnd2 Mouse)
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-Ec.coaE (pESC124)

   Plasmid

   #50390

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   DPCK
   

   Tags

   6XHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rescue Septin 11 mRNA

    Plasmid

    #122022

    Purpose

    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Expression

    Mammalian

    Mutation

    Silent mutations are in 5 consecutive codons (AUU…

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESC-NAT-LACap

    Plasmid

    #80763

    Purpose

    Expresses truncated L-A capsid for curing satellite virus

    Depositor

    John McCusker

    Insert

    L-A cDNA (L-A Cap)
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. peSCBE3-NG-HF1-Hypa

    Plasmid

    #218166

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

17. Fluorescent reporter for MOBE3-4

    Plasmid

    #219946

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pBluescript-mouse eIF2C2

    Plasmid

    #50354

    Purpose

    Mouse eIF2C2 cDNA clone

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C2 (Ago2 Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pESC-LEU-HsAIDSc

    Plasmid

    #60810

    Purpose

    to express human AID recoded for yeast codon usage

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

    Insert

    AID (AICDA Human)
    

    Tags

    Myc

    Expression

    Yeast

    Mutation

    codon optimized for yeast

    Promoter

    GAL1

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only