We narrowed to 12,515 results for: NSI
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Bacterial Strain#197655PurposeThis strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.DepositorBacterial ResistanceNoneAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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EC1000
Bacterial Strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial ResistanceKanamycinAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATMW1
Bacterial Strain#218768PurposeE. coli strain, genotype: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galKDepositorBacterial ResistanceSpectinomycin (50 ug/mL), Gentamycin (10 ug/mL)SpeciesEscherichia coliAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
TagsYFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout Library with UMIs
Pooled Library#221409PurposegRNA library to test the impact of transporters on biological processesDepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeExpansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into Saccharomyces cerevisiae by CRISPR/Cas9.DepositorApplicationCloning and Synthetic BiologyVector TypeYeast ExpressionCloning TypeUSERAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
OVC Extended Collection
Plasmid Kit#1000000165PurposeExpansion kit for #1000000164 includes alternative linker configurations in site B and light-activated Opto-caspase 9 constructs.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeMammalian ExpressionCloning TypeRestriction EnzymeAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human SLC overexpression library
Pooled Library#213694PurposeInducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human ABC Transporter Knockout Sub-library with UMIs
Pooled Library#221410PurposegRNA library to test the impact of transporters on biological processes (subset: ABC transporters and controls)DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
KSHV Tiling Library
Pooled Library#180272PurposeTiles the entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes Virus genome. A guide every ~8 bp allows pooled screening of the viral genome.DepositorExpressionMammalianSpeciesKaposi’s sarcoma-associated herpes virusUseLentiviralAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Alexandrov Cell-Free Protein Expression Kit (PEK)
Plasmid Kit#1000000065PurposePlasmids consisting of open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system.DepositorApplicationGene Expression and LabelingVector TypeIn Vitro ExpressionAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS9
Plasmid#111765PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS5
Plasmid#111763PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mouse Chromatin Regulator Library
Pooled Library#200011PurposeKnockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM4731
Plasmid#85127PurposeNSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.DepositorInsertPkaiBC-KaiB
ExpressionBacterialAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-G2304D
Plasmid#182845Purposemutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sitesDepositorAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-EGFP-6H
Plasmid#187001PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only