We narrowed to 11,841 results for: NSI

1. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

2. EC1000

   Bacterial Strain

   #71852

   Purpose

   RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

   Depositor

   Jan Kok, Todd Klaenhammer

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Bacterial Resistance

   Kanamycin

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. ATMW1

   Bacterial Strain

   #218768

   Purpose

   E. coli strain, genotype: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Bacterial Resistance

   Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

   Species

   Escherichia coli

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pAM5081

   Plasmid

   #85107

   Purpose

   Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.

   Depositor

   Susan Golden

   Article

   Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

   Insert

   YFP-KaiC
   

   Use

   Tags

   YFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human Transporter Knockout Library with UMIs

   Pooled Library

   #221409

   Purpose

   gRNA library to test the impact of transporters on biological processes

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

6. Human SLC overexpression library

   Pooled Library

   #213694

   Purpose

   Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

7. Human ABC Transporter Knockout Sub-library with UMIs

   Pooled Library

   #221410

   Purpose

   gRNA library to test the impact of transporters on biological processes (subset: ABC transporters and controls)

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

8. KSHV Tiling Library

   Pooled Library

   #180272

   Purpose

   sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

   Depositor

   Britt Glaunsinger

   Article

   Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

   Expression

   Mammalian

   Species

   Kaposi’s sarcoma-associated herpes virus

   Use

   Lentiviral

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

9. Mouse Chromatin Regulator Library

   Pooled Library

   #200011

   Purpose

   CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

   Depositor

   Robert Manguso, Bradley Bernstein, Gabriel Griffin

   Article

   Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

10. Mouse Validation (mVAL) CRISPR Library

    Pooled Library

    #159391

    Purpose

    Pooled CRISPR library targets core intrinsic immune evasion genes

    Depositor

    Jason Moffat

    Article

    Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

11. VinTS-tCRMod-GGSGGS9

    Plasmid

    #111765

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VinTS-tCRMod-GGSGGS5

    Plasmid

    #111763

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Phat2-dr-cPla2-C2-EGFP-6H

    Plasmid

    #187001

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Phat2-dr-cPla2-C2-mCherry-6H

    Plasmid

    #187104

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

    Plasmid

    #187186

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Y90F, V91F

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tCRMod-GGSGGS9

    Plasmid

    #111762

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS9-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tCRMod-GGSGGS7

    Plasmid

    #111761

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS7-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge