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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 101 - 120 of 6562 results

  1. xCas9(3.7)-BE4

    Plasmid
    #108381
    Purpose
    Mammalian expression vector for xCas9(3.7)-BE4
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.7)-BE4
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    108381-plasmid-map-sequence-id-217618

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  5. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42337
    Purpose
    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42337-plasmid-map-sequence-id-57166

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  6. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid
    #59987
    Purpose
    Eef1a (EF1alpha) promoter driving nls::Cas9::nls
    Depositor
    Lionel Christiaen
    Publication
    Stolfi et al Development. 2014 Nov;141(21):4115-20. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nls::Cas9::nls
    Use
    CRISPR
    Mutation
    Reverted mutations from dCas9 to wiltdtype
    Promoter
    Ciinte.Eef1a (EF1alpha) -1955/-1
    Available since
    Nov. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    59987-plasmid-map-sequence-id-95666

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  17. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Publication
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    46963-plasmid-map-sequence-id-66854

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  20. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    190034-plasmid-map-sequence-id-371641

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Showing: 101 - 120 of 6562 results