We narrowed to 7,721 results for: nls

1. pBPnlsLexA::p65Uw

   Plasmid

   #26230

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::p65 (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Use

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNLS-iRFP720

   Plasmid

   #45467

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-NLS-HA-B3

   Plasmid

   #51270

   Purpose

   Expression of the site-specific recombinase B3 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-B3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ (M)-HT-NLS

   Plasmid

   #82518

   Purpose

   Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Nuclear localizaition sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

   Plasmid

   #81251

   Purpose

   Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-nlsGFP no-pA

   Plasmid

   #80815

   Purpose

   nlsGFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAi14-GFPNLS-MC

   Plasmid

   #87114

   Purpose

   minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFPNLSPpA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   nEF

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR'CMVGFP_NLS_hRIF1(1-1308)

    Plasmid

    #23134

    Depositor

    Lifeng Xu

    Article

    Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

    Insert

    RIF1 deletion construct (RIF1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPT-nlsLacZ

    Plasmid

    #12261

    Depositor

    Didier Trono

    Insert

    LacZ
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVMagFRET-6-NLS

    Plasmid

    #50757

    Purpose

    Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-6 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-2a-6MTnlstdTomato

    Plasmid

    #67699

    Purpose

    3' entry clone with 6xmyc nuclear localized tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    tdTomato
    

    Use

    Tags

    2A-6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. R26/P-nlsrtTA

    Plasmid

    #46183

    Depositor

    Rudolf Jaenisch

    Article

    Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

    Inserts

    nlsrtTA
    pgk-puro
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

17. NLS-GAI(1-92)

    Plasmid

    #37317

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

18. p3E-CMVmin:nlsGFP pA

    Plasmid

    #80821

    Purpose

    CMVmin-promoted nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-nlsGFP pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVmin

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLS-LSSmKate1-N1

    Plasmid

    #31870

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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