We narrowed to 7,697 results for: nls

1. p3E-nlsGFP no-pA

   Plasmid

   #80815

   Purpose

   nlsGFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMVMagFRET-6-NLS

   Plasmid

   #50757

   Purpose

   Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-6 with NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-2a-6MTnlstdTomato

   Plasmid

   #67699

   Purpose

   3' entry clone with 6xmyc nuclear localized tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   tdTomato
   

   Use

   Tags

   2A-6xMyc-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. R26/P-nlsrtTA

   Plasmid

   #46183

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

   Inserts

   nlsrtTA
   pgk-puro
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

6. NLS-GAI(1-92)

   Plasmid

   #37317

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

7. p3E-CMVmin:nlsGFP pA

   Plasmid

   #80821

   Purpose

   CMVmin-promoted nlsGFP with pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVmin

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNLS-LSSmKate1-N1

   Plasmid

   #31870

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   CMV

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNLS-LSSmKate2-N1

   Plasmid

   #31871

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Promoter

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Use

    Tags

    2A and 6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LI B-C N-NLS KOZ c2

    Plasmid

    #54443

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    Nuclear localization signal (NLS) of SV40 large T antigen
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCNL-N1_3xNLS

    Plasmid

    #65673

    Purpose

    Expresses NLS-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3E-nlsGFP pA

    Plasmid

    #80814

    Purpose

    nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    nlsGFPpA 3'E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYNL-N1_3xNLS

    Plasmid

    #65672

    Purpose

    Expresses NLS-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pONL-N1_3xNLS

    Plasmid

    #65674

    Purpose

    Expresses NLS-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His_CL7_2NLS_iCas12a-4NLS

    Plasmid

    #222971

    Purpose

    Bacterial expression of NLS-rich iCas12a construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His_CL7_2NLS_iCas12a-4NLS
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid

    #197031

    Purpose

    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized denOsCas12f1-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R / D228A / D406A

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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