We narrowed to 2,375 results for: pcas9

1. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEC1.2/EC1.1_spCas9_TagRFP

   Plasmid

   #161934

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO5d.EFS.SpCas9.P2A.BSD

   Plasmid

   #57821

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC1.2/EC1.1_spCas9_GFP

   Plasmid

   #161933

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA-SpCas9-g3

   Plasmid

   #80786

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+hSpCas9

   Plasmid

   #51815

   Purpose

   Encode hSpCas9 for in vitro transcription with Sp6

   Depositor

   Masato Kinoshita

   Article

   Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

   Insert

   humanized S.pyogenes Cas9
   

   Use

   CRISPR; Medaka, zebrafish

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-HE-Geminin

    Plasmid

    #109402

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HE-Geminin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-spCas9-SB

    Plasmid

    #169919

    Purpose

    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    SpCas9-SB100X
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA-SpCas9-g2

    Plasmid

    #80785

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p276 eSpCas9_2gRNAs_hH11

    Plasmid

    #164850

    Purpose

    gRNA vector for targeting human H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

    Insert

    gRNAs for targeting human H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-CBE6a

    Plasmid

    #215819

    Purpose

    Express CBE6a (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVNP2.0-SpCas9

    Plasmid

    #206880

    Purpose

    Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    SpCas9 (cas9 )
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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