Skip to main content
Addgene
Showing: 1181 - 1200 of 32575 results

  1. PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein

    Plasmid
    #200297
    Purpose
    Bacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 Metallothionein
    Depositor
    Artur Krężel
    Article
    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.
    Insert
    Gene of metallothionein with Chiting Binding Domanin-Intein
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200297-plasmid-map-sequence-id-394920

    Enlarge

  4. SpMTA Purple Sea Urchin Metallothionein

    Plasmid
    #200292
    Purpose
    Bacterial expression plasmids for production of recombinant Purple Sea Urchin Strongylocentrotus purpuratus Metallothionein
    Depositor
    Artur Krężel
    Article
    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.
    Insert
    Gene of metallothionein with Chiting Binding Domanin-Intein
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    200292-plasmid-map-sequence-id-394816

    Enlarge

  5. AAVS1-TRE3G-NODALvar-matMYC

    Plasmid
    #115641
    Purpose
    For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115641-plasmid-map-sequence-id-232862

    Enlarge

  7. pEPKK0CM0480

    Plasmid
    #177037
    Purpose
    Level 0 promoter binding site part for MoClo assembly, nonstandard overlaps, CCAT-TACT
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177037-plasmid-map-sequence-id-349006

    Enlarge

  11. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

    Enlarge

  12. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

    Enlarge

  15. pHL1017

    Plasmid
    #62828
    Purpose
    Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Insert
    gfp
    Use
    Synthetic Biology
    Tags
    m8::glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pHL1757

    Plasmid
    #53037
    Purpose
    PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    PconNoHindM2 and pConNoHind
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-Ef1a-fDIO-JRGECO1a (AAV1)

    Viral Prep
    #128317-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Patricia Jensen
    Promoter
    EF1a
    Tags
    None
    Available since
    March 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

    Enlarge

  20. CMV-G-GECO1.2

    Plasmid
    #32446
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    G-GECO1.2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32446-plasmid-map-sequence-id-122736

    Enlarge

Showing: 1181 - 1200 of 32575 results